Anti-S100A14 Antibody (CAB10394)
- SKU:
- CAB10394
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-S100A14 Antibody |
| Antibody SKU: | CAB10394 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human S100A14 (NP_065723.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BxPC-3, Mouse lung, Mouse thymus, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human S100A14 (NP_065723.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGQC RSAN AEDA QEFS DVER AIET LIKN FHQY SVEG GKET LTPS ELRD LVTQ QLPH LMPS NCGL EEKI ANLG SCND SKLE FRSF WELI GEAA KSVK LERP VRGH |
| Gene ID: | 57402 |
| Uniprot: | Q9HCY8 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 11kDa |
| Observed MW: | 11kDa |
| Synonyms: | S100A14, BCMP84, S100A15 |
| Background: | This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). |
| UniProt Protein Function: | S100A14: a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011] |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: cell junction; cytosol; nuclear body; perinuclear region of cytoplasm Molecular Function:chemokine receptor binding; protein binding Biological Process: defense response to bacterium; response to lipopolysaccharide; toll-like receptor 4 signaling pathway |
| NCBI Summary: | This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q9HCY8 |
| NCBI GenInfo Identifier: | 20178118 |
| NCBI Gene ID: | 57402 |
| NCBI Accession: | Q9HCY8.1 |
| UniProt Secondary Accession: | Q9HCY8,Q5RHT0, |
| UniProt Related Accession: | Q9HCY8 |
| Molecular Weight: | 12kDa |
| NCBI Full Name: | Protein S100-A14 |
| NCBI Synonym Full Names: | S100 calcium binding protein A14 |
| NCBI Official Symbol: | S100A14 |
| NCBI Official Synonym Symbols: | BCMP84; S100A15 |
| NCBI Protein Information: | protein S100-A14 |
| UniProt Protein Name: | Protein S100-A14 |
| UniProt Synonym Protein Names: | S100 calcium-binding protein A14; S114 |
| Protein Family: | Protein |
| UniProt Gene Name: | S100A14 |
 | Western blot analysis of extracts of various cell lines, using S100A14 antibody (CAB10394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human esophageal using S100A14 Rabbit pAb (CAB10394) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using S100A14 Rabbit pAb (CAB10394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using S100A14 Rabbit pAb (CAB10394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using S100A14 Rabbit pAb (CAB10394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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