Anti-RUFY3 Antibody (CAB12896)

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SKU:
CAB12896
Product type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-RUFY3 Antibody
Antibody SKU:CAB12896
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human RUFY3 (NP_055776.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-65 of human RUFY3 (NP_055776.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAL TPPT DMPT PTTD KITQ AAME TIYL CKFR VSMD GEWL CLRE LDDI SLTP DPEP THED PNYL M
Gene ID:22902
Uniprot:Q7L099
Cellular Location:Cell projection, Cytoplasm, Endomembrane system, Perikaryon, filopodium, growth cone, invadopodium, lamellipodium
Calculated MW:52kDa/55kDa/64kDa/70kDa
Observed MW:56kDa
Synonyms:RUFY3, RIPX, SINGAR1, ZFYVE30
Background:This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RUFY3: Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: axon; cytoplasm; cytosol; dendrite; filopodium; growth cone; lamellipodium; perikaryon

Molecular Function:protein binding

Biological Process: actin filament organization; negative regulation of axonogenesis; positive regulation of axon extension; positive regulation of cell migration

NCBI Summary:This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
UniProt Code:Q7L099
NCBI GenInfo Identifier:74738521
NCBI Gene ID:22902
NCBI Accession:Q7L099.1
UniProt Secondary Accession:Q7L099,O94948, Q9UI00, B3KM25, B4DYW7, D9N163,
UniProt Related Accession:Q7L099
Molecular Weight:53kDa
NCBI Full Name:Protein RUFY3
NCBI Synonym Full Names:RUN and FYVE domain containing 3
NCBI Official Symbol:RUFY3  
NCBI Official Synonym Symbols:RIPX; SINGAR1; ZFYVE30  
NCBI Protein Information:protein RUFY3
UniProt Protein Name:Protein RUFY3
UniProt Synonym Protein Names:RUN and FYVE domain-containing protein 3
Protein Family:Protein
UniProt Gene Name:RUFY3  
Anti-RUFY3 Antibody (CAB12896)Western blot analysis of extracts of various cell lines, using RUFY3 antibody (CAB12896) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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