Anti-Rubicon Antibody (CAB13169)
- SKU:
- CAB13169
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-Rubicon Antibody |
| Antibody SKU: | CAB13169 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRPE GAGM ELGG GEER LPEE SRRE HWQL LGNL KTTV EGLV STNS PNVW SKYG GLER LCRD MQSI LYHG LIRD QACR RQTD YWQF VKDI RWLS PHSA LHVE KFIS VHEN DQSS ADGA SERA VAEL WLQH SLQY HCLS AQLR PLLG DRQY IRKF YTDA AFLL SDAH VTAM LQCL EAVE QNNP RLLA QIDA SMFA RKHE SPLL |
| Gene ID: | 9711 |
| Uniprot: | Q92622 |
| Cellular Location: | Early endosome, Late endosome, Lysosome |
| Calculated MW: | 102kDa/103kDa/108kDa |
| Observed MW: | 108kDa |
| Synonyms: | RUBCN, KIAA0226, RUBICON, SCAR15 |
| Background: | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| UniProt Protein Function: | rubicon: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity. Forms a complex with BECN1, PIK3C3, PIK3R4 and UVRAG, but not with ATG14. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 3q29 Cellular Component: cytoplasm; early endosome; Golgi apparatus; intracellular membrane-bound organelle; late endosome; nucleoplasm Molecular Function:protein binding Biological Process: negative regulation of autophagy; negative regulation of endocytosis; negative regulation of phosphoinositide 3-kinase activity Disease: Spinocerebellar Ataxia, Autosomal Recessive 15 |
| NCBI Summary: | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q92622 |
| NCBI GenInfo Identifier: | 296439479 |
| NCBI Gene ID: | 9711 |
| NCBI Accession: | Q92622.4 |
| UniProt Secondary Accession: | Q92622,Q96CK5, |
| UniProt Related Accession: | Q92622 |
| Molecular Weight: | 102,506 Da |
| NCBI Full Name: | Run domain Beclin-1-interacting and cysteine-rich domain-containing protein |
| NCBI Synonym Full Names: | RUN and cysteine rich domain containing beclin 1 interacting protein |
| NCBI Official Symbol: | RUBCN |
| NCBI Official Synonym Symbols: | SCAR15; RUBICON; KIAA0226 |
| NCBI Protein Information: | run domain Beclin-1-interacting and cysteine-rich domain-containing protein |
| UniProt Protein Name: | Run domain Beclin-1-interacting and cysteine-rich domain-containing protein |
| UniProt Synonym Protein Names: | Beclin-1 associated RUN domain containing protein; Baron |
| UniProt Gene Name: | RUBCN |
| UniProt Entry Name: | RUBIC_HUMAN |
 | Western blot analysis of extracts of mouse brain, using Rubicon antibody (CAB13169) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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