Anti-RTKN Antibody (CAB6732)
- SKU:
- CAB6732
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-RTKN Antibody |
| Antibody SKU: | CAB6732 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-431, HepG2, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 254-513 of human RTKN (NP_001015056.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PLCM TQPT ASGT LRVQ QAGE MQNW AQVH GVLK GTNL FCYR QPED ADTG EEPL LTIA VNKE TRVR AGEL DQAL GRPF TLSI SNQY GDDE VTHT LQTE SREA LQSW MEAL WQLF FDMS QWKQ CCDE IMKI ETPA PRKP PQAL AKQG SLYH EMAI EPLD DIAA VTDI LTQR EGAR LETP PPWL AMFT DQPA LPNP CSPA SVAP APDW THPL PWGR PRTF SLDA VPPD HSPR ARSV APLP PQRS PRTR GLCS KGQP RTWL QSPV |
| Gene ID: | 6242 |
| Uniprot: | Q9BST9 |
| Cellular Location: | |
| Calculated MW: | 56kDa/61kDa/62kDa |
| Observed MW: | 63kDa |
| Synonyms: | RTKN, rhotekin |
| Background: | This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | RTKN: Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein regulator, misc. Chromosomal Location of Human Ortholog: 2p13.1 Molecular Function:protein binding; GTP binding; GTP-Rho binding; GTPase inhibitor activity Biological Process: apoptosis; negative regulation of catalytic activity; signal transduction; Rho protein signal transduction |
| NCBI Summary: | This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9BST9 |
| NCBI GenInfo Identifier: | 74733052 |
| NCBI Gene ID: | 6242 |
| NCBI Accession: | Q9BST9.2 |
| UniProt Secondary Accession: | Q9BST9,Q8WVN1, Q96PT6, Q9HB05, H7BXD4, |
| UniProt Related Accession: | Q9BST9 |
| Molecular Weight: | 563 |
| NCBI Full Name: | Rhotekin |
| NCBI Synonym Full Names: | rhotekin |
| NCBI Official Symbol: | RTKN |
| NCBI Protein Information: | rhotekin; RTKN |
| UniProt Protein Name: | Rhotekin |
| Protein Family: | Rhotekin |
| UniProt Gene Name: | RTKN |
| UniProt Entry Name: | RTKN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RTKN antibody (CAB6732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of MCF7 cells using RTKN antibody (CAB6732). Blue: DAPI for nuclear staining. |
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