Anti-RRBP1 Antibody (CAB12239)

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SKU:
CAB12239
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-RRBP1 Antibody
Antibody SKU:CAB12239
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human RRBP1 (NP_004578.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human RRBP1 (NP_004578.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDIY DTQT LGVV VFGG FMVV SAIG IFLV STFS MKET SYEE ALAN QRKE MAKT HHQK VEKK KKEK TVEK KGKT KKKE EKPN GKIP DHDP APNV TVLL REPV RAPA VAVA PTPV QPPI IVAP VATV PAMP QEKL ASSP KDKK
Gene ID:6238
Uniprot:Q9P2E9
Cellular Location:Endoplasmic reticulum membrane, Single-pass type III membrane protein
Calculated MW:108kDa/152kDa
Observed MW:120kDa
Synonyms:RRBP1, ES/130, ES130, RRp, hES
Background:This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12.
UniProt Protein Function:RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; membrane; ribosome

Molecular Function:receptor activity

Biological Process: osteoblast differentiation; translation

NCBI Summary:This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]
UniProt Code:Q9P2E9
NCBI GenInfo Identifier:23822112
NCBI Gene ID:6238
NCBI Accession:Q9P2E9.4
UniProt Secondary Accession:Q9P2E9,O75300, O75301, Q5W165, Q96SB2, Q9BWP1, Q9H476 A2A2S6, A6NCN6,
UniProt Related Accession:Q9P2E9
Molecular Weight:108,632 Da
NCBI Full Name:Ribosome-binding protein 1
NCBI Synonym Full Names:ribosome binding protein 1
NCBI Official Symbol:RRBP1  
NCBI Official Synonym Symbols:RRp; hES; ES130; ES/130  
NCBI Protein Information:ribosome-binding protein 1
UniProt Protein Name:Ribosome-binding protein 1
UniProt Synonym Protein Names:180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein
Protein Family:Ribosome-binding protein
UniProt Gene Name:RRBP1  
UniProt Entry Name:RRBP1_HUMAN
Anti-RRBP1 Antibody (CAB12239)Western blot analysis of extracts of various cell lines, using RRBP1 antibody (CAB12239) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RRBP1 Antibody (CAB12239)Confocal immunofluorescence analysis of HeLa cells using RRBP1 Polyclonal Antibody (CAB12239) at dilution of 1:100. Blue: DAPI for nuclear staining.

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