Anti-RRAGA Antibody (CAB7771)
- SKU:
- CAB7771
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-RRAGA Antibody |
| Antibody SKU: | CAB7771 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human RRAGA (NP_006561.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human RRAGA (NP_006561.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPNT AMKK KVLL MGKS GSGK TSMR SIIF ANYI ARDT RRLG ATID VEHS HVRF LGNL VLNL WDCG GQDT FMEN YFTS QRDN IFRN VEVL IYVF DVES RELE KDMH YYQS CLEA ILQN SPDA KIFC LVHK MDLV QEDQ RDLI FKER EEDL RRLS RPLE CACF RTSI WDET LYKA WSSI VYQL IPNV QQLE MNLR NFAQ IIEA DEVL LFER ATFL VISH YQCK EQRD VHRF EKIS NIIK QFKL SCSK LAAS FQSM EVRN SNFA AFID IFTS NTYV MVVM SDPS IPSA ATLI NIRN ARKH FEKL ERVD GPKH SLLM R |
| Gene ID: | 10670 |
| Uniprot: | Q7L523 |
| Cellular Location: | Cytoplasm, Lysosome, Nucleus |
| Calculated MW: | 36kDa |
| Observed MW: | 33KDa |
| Synonyms: | RRAGA, FIP-1, FIP1, RAGA |
| Background: |
| UniProt Protein Function: | RRAGA: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF- alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts with adenovirus E3 14.7 kDa protein. Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. Belongs to the GTR/RAG GTP-binding protein family. |
| UniProt Protein Details: | Protein type:G protein regulator, misc. Chromosomal Location of Human Ortholog: 9p22.1 Cellular Component: cytoplasm; cytosol; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; nucleus Molecular Function:GTP binding; GTPase activity; phosphoprotein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; ubiquitin protein ligase binding Biological Process: apoptosis; cell cycle arrest; cell death; cellular response to amino acid starvation; gene expression; insulin receptor signaling pathway; macroautophagy; negative regulation of autophagy; positive regulation of cytolysis; positive regulation of TOR signaling pathway; transcription initiation from RNA polymerase II promoter; virus-host interaction |
| UniProt Code: | Q7L523 |
| NCBI GenInfo Identifier: | 74759007 |
| NCBI Gene ID: | 10670 |
| NCBI Accession: | Q7L523.1 |
| UniProt Secondary Accession: | Q7L523,O00290, Q15347, B2R7L1, |
| UniProt Related Accession: | Q7L523 |
| Molecular Weight: | 36,566 Da |
| NCBI Full Name: | Ras-related GTP-binding protein A |
| NCBI Synonym Full Names: | Ras related GTP binding A |
| NCBI Official Symbol: | RRAGA |
| NCBI Official Synonym Symbols: | FIP1; RAGA; FIP-1 |
| NCBI Protein Information: | ras-related GTP-binding protein A |
| UniProt Protein Name: | Ras-related GTP-binding protein A |
| UniProt Synonym Protein Names: | Adenovirus E3 14.7 kDa-interacting protein 1 |
| UniProt Gene Name: | RRAGA |
| UniProt Entry Name: | RRAGA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RRAGA antibody (CAB7771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using RRAGA Rabbit pAb (CAB7771) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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