Anti-RPL27 항체 (CAB13044)

Antibody Name:	Anti-RPL27 Antibody
Antibody SKU:	CAB13044
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A-431, 293T, LO2, Mouse liver, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKF MKPG KVVL VLAG RYSG RKAV IVKN IDDG TSDR PYSH ALVA GIDR YPRK VTAA MGKK KIAK RSKI KSFV KVYN YNHL MPTR YSVD IPLD KTVV NKDV FRDP ALKR KARR EAKV KFEE RYKT GKNK WFFQ KLRF
Gene ID:	6155
Uniprot:	P61353
Cellular Location:
Calculated MW:	15kDa
Observed MW:	16kDa

Synonyms:

RPL27, L27

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL27: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 17q21 Cellular Component: focal adhesion; membrane; ribosome; nucleus; ribonucleoprotein complex; cytosol Molecular Function:structural constituent of ribosome Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P61353
NCBI GenInfo Identifier:	47117772
NCBI Gene ID:	6155
NCBI Accession:	P61353.2
UniProt Secondary Accession:	P61353,P08526, Q4G0A9,
UniProt Related Accession:	P61353
Molecular Weight:	136
NCBI Full Name:	60S ribosomal protein L27
NCBI Synonym Full Names:	ribosomal protein L27
NCBI Official Symbol:	RPL27
NCBI Official Synonym Symbols:	L27
NCBI Protein Information:	60S ribosomal protein L27
UniProt Protein Name:	60S ribosomal protein L27
Protein Family:	54S ribosomal protein
UniProt Gene Name:	RPL27
UniProt Entry Name:	RL27_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL27 antibody (CAB13044) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using RPL27 Polyclonal Antibody (CAB13044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using RPL27 Polyclonal Antibody (CAB13044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RPL27 Polyclonal Antibody (CAB13044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of HeLa cells using RPL27 Polyclonal Antibody (CAB13044) at dilution of 1:200. Blue: DAPI for nuclear staining.

Antibodies

RPL27 Antibody (PACO11920)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)