Anti-RPL21 Antibody (CAB14254)
- SKU:
- CAB14254
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RPL21 Antibody |
| Antibody SKU: | CAB14254 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, Raji, BxPC-3, DU145, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTNT KGKR RGTR YMFS RPFR KHGV VPLA TYMR IYKK GDIV DIKG MGTV QKGM PHKC YHGK TGRV YNVT QHAV GIVV NKQV KGKI LAKR INVR IEHI KHSK SRDS FLKR VKEN DQKK KEAK EKGT WVQL KRQP APPR EAHF VRTN GKEP ELLE PIPY EFMA |
| Gene ID: | 6144 |
| Uniprot: | P46778 |
| Cellular Location: | |
| Calculated MW: | 18kDa |
| Observed MW: | 18kDa |
| Synonyms: | RPL21, HYPT12, L21 |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL21: Defects in RPL21 are a cause of generalized hypotrichosis simplex (HTS). A rare form of non-syndromic hereditary hypotrichosis without characteristic hair shaft anomalies. Affected individuals typically show normal hair at birth, but hair loss and thinning of the hair shaft start during early childhood and progress with age. Belongs to the ribosomal protein L21e family. |
| UniProt Protein Details: | Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 13q12.2 Cellular Component: membrane; cytoplasm; nucleolus; cytosol Molecular Function:structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle Disease: Hypotrichosis 12 |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P46778 |
| NCBI GenInfo Identifier: | 18104948 |
| NCBI Gene ID: | 6144 |
| NCBI Accession: | NP_000973.2 |
| UniProt Secondary Accession: | P46778,Q16699, |
| UniProt Related Accession: | P46778 |
| Molecular Weight: | 18,565 Da |
| NCBI Full Name: | 60S ribosomal protein L21 |
| NCBI Synonym Full Names: | ribosomal protein L21 |
| NCBI Official Symbol: | RPL21 |
| NCBI Official Synonym Symbols: | L21; HYPT12 |
| NCBI Protein Information: | 60S ribosomal protein L21 |
| UniProt Protein Name: | 60S ribosomal protein L21 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL21 |
| UniProt Entry Name: | RL21_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL21 antibody (CAB14254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RPL21 antibody (CAB14254) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of HeLa cells using RPL21 antibody (CAB14254) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of A-431 cells using RPL21 antibody (CAB14254) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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