Anti-RPL14 항체 (CAB6724)

Antibody Name:	Anti-RPL14 Antibody
Antibody SKU:	CAB6724
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, HeLa, 293T, Jurkat, HepG2, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVFR RFVE VGRV AYVS FGPH AGKL VAIV DVID QNRA LVDG PCTQ VRRQ AMPF KCMQ LTDF ILKF PHSA HQKY VRQA WQKA DINT KWAA TRWA KKIE ARER KAKM TDFD RFKV MKAK KMRN RIIK NEVK KLQK AALL KASP KKAP GTKG TAAA AAAA AAAK VPAK KITA ASKK APAQ KVPA QKAT GQKA APAP KAQK GQKA PAQK APAP KASG KKA
Gene ID:	9045
Uniprot:	P50914
Cellular Location:
Calculated MW:	23kDa
Observed MW:	26kDa

Synonyms:

RPL14, CAG-ISL-7, CTG-B33, L14, RL14, hRL14

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL14: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 3p22-p21.2 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; rRNA processing
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P50914
NCBI GenInfo Identifier:	212276521
NCBI Gene ID:	9045
NCBI Accession:	P50914.4
UniProt Related Accession:	P50914
Molecular Weight:	23kDa
NCBI Full Name:	60S ribosomal protein L14
NCBI Synonym Full Names:	ribosomal protein L14
NCBI Official Symbol:	RPL14
NCBI Official Synonym Symbols:	L14; RL14; hRL14; CTG-B33; CAG-ISL-7
NCBI Protein Information:	60S ribosomal protein L14
UniProt Protein Name:	60S ribosomal protein L14
UniProt Synonym Protein Names:	CAG-ISL 7
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL14
UniProt Entry Name:	RL14_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL14 antibody (CAB6724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat liver using RPL14 antibody (CAB6724) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using RPL14 antibody (CAB6724). Blue: DAPI for nuclear staining.

Antibodies

Anti-RPL14 Antibody (CAB13384)

RPL14 Antibody (PACO05670)

RPL14 Antibody (PACO11911)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)