Anti-RPAP3 Antibody (CAB15239)
- SKU:
- CAB15239
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RPAP3 Antibody |
| Antibody SKU: | CAB15239 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, 293T, HT-29, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NAEE LQDF MRDL ENWE KDIK QKDM ELRR QNGV PEEN LPPI RNGN FRKK KKGK AKES SKKT REEN TKNR IKSY DYEA WAKL DVDR ILDE LDKD DSTH ESLS QESE SEED GIHV DSQK ALVL KEKG NKYF KQGK YDEA IDCY TKGM DADP YNPV LPTN RASA YFRL KKFA VAES DCNL AVAL NRSY TKAY SRRG AARF ALQK LEEA KKDY ERVL ELEP NNFE ATNE L |
| Gene ID: | 79657 |
| Uniprot: | Q9H6T3 |
| Cellular Location: | |
| Calculated MW: | 57kDa/71kDa/75kDa |
| Observed MW: | 76kDa |
| Synonyms: | RPAP3, hSpagh |
| Background: | This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | RPAP3: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Belongs to the RPAP3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 12q13.11 Molecular Function:protein binding |
| NCBI Summary: | This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009] |
| UniProt Code: | Q9H6T3 |
| NCBI GenInfo Identifier: | 158564023 |
| NCBI Gene ID: | 79657 |
| NCBI Accession: | Q9H6T3.2 |
| UniProt Secondary Accession: | Q9H6T3,Q6PHR5, B4DRW9, |
| UniProt Related Accession: | Q9H6T3 |
| Molecular Weight: | 57,108 Da |
| NCBI Full Name: | RNA polymerase II-associated protein 3 |
| NCBI Synonym Full Names: | RNA polymerase II associated protein 3 |
| NCBI Official Symbol: | RPAP3 |
| NCBI Protein Information: | RNA polymerase II-associated protein 3 |
| UniProt Protein Name: | RNA polymerase II-associated protein 3 |
| Protein Family: | RNA polymerase II-associated protein |
| UniProt Gene Name: | RPAP3 |
| UniProt Entry Name: | RPAP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPAP3 antibody (CAB15239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using RPAP3 antibody (CAB15239) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using RPAP3 antibody (CAB15239) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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