Anti-ROS1 Antibody (CAB16669)
- SKU:
- CAB16669
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ROS1 Antibody |
| Antibody SKU: | CAB16669 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2100-2200 of human ROS1 (NP_002935.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 2100-2200 of human ROS1 (NP_002935.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IGDF GLAR DIYK NDYY RKRG EGLL PVRW MAPE SLMD GIFT TQSD VWSF GILI WEIL TLGH QPYP AHSN LDVL NYVQ TGGR LEPP RNCP DDLW NLMT QCWA Q |
| Gene ID: | 6098 |
| Uniprot: | P08922 |
| Cellular Location: | |
| Calculated MW: | 263kDa |
| Observed MW: | 263kDa |
| Synonyms: | ROS1, MCF3, ROS, c-ros-1 |
| Background: | This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. |
| UniProt Protein Function: | ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma. |
| UniProt Protein Details: | Protein type:Kinase, protein; Membrane protein, integral; Protein kinase, TK; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Sev family Chromosomal Location of Human Ortholog: 6q22 Cellular Component: membrane Molecular Function:protein binding; protein phosphatase binding; protein-tyrosine kinase activity Biological Process: cell differentiation; cell growth; cell proliferation; columnar/cuboidal epithelial cell development; protein amino acid phosphorylation; regulation of TOR signaling pathway; spermatogenesis |
| NCBI Summary: | This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P08922 |
| NCBI GenInfo Identifier: | 126302596 |
| NCBI Gene ID: | 6098 |
| NCBI Accession: | P08922.3 |
| UniProt Secondary Accession: | P08922,Q15368, Q5TDB5, |
| UniProt Related Accession: | P08922 |
| Molecular Weight: | 263,915 Da |
| NCBI Full Name: | Proto-oncogene tyrosine-protein kinase ROS |
| NCBI Synonym Full Names: | ROS proto-oncogene 1, receptor tyrosine kinase |
| NCBI Official Symbol: | ROS1 |
| NCBI Official Synonym Symbols: | ROS; MCF3; c-ros-1 |
| NCBI Protein Information: | proto-oncogene tyrosine-protein kinase ROS |
| UniProt Protein Name: | Proto-oncogene tyrosine-protein kinase ROS |
| UniProt Synonym Protein Names: | Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase |
| Protein Family: | Rosmarinate synthase |
| UniProt Gene Name: | ROS1 |
| UniProt Entry Name: | ROS1_HUMAN |
 | Western blot analysis of extracts of mouse brain, using ROS1 antibody (CAB16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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