Anti-ROCK2 Antibody (CAB5698)
- SKU:
- CAB5698
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ROCK2 Antibody |
| Antibody SKU: | CAB5698 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1388 of human ROCK2 (NP_004841.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HT-29, ES-2, U-937, U-87MG, Mouse lung, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1109-1388 of human ROCK2 (NP_004841.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LDSK DSDI EQLR SQLQ ALHI GLDS SSIG SGPG DAEA DDGF PESR LEGW LSLP VRNN TKKF GWVK KYVI VSSK KILF YDSE QDKE QSNP YMVL DIDK LFHV RPVT QTDV YRAD AKEI PRIF QILY ANEG ESKK EQEF PVEP VGEK SNYI CHKG HEFI PTLY HFPT NCEA CMKP LWHM FKPP PALE CRRC HIKC HKDH MDKK EEII APCK VYYD ISTA KNLL LLAN STEE QQKW VSRL VKKI PKKP PAPD PFAR SSPR TSMK IQQN QSIR RPSR QLAP NKPS |
| Gene ID: | 9475 |
| Uniprot: | O75116 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 160kDa |
| Observed MW: | 180kDa |
| Synonyms: | ROCK2, ROCK-II |
| Background: | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. |
| UniProt Protein Function: | ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily Chromosomal Location of Human Ortholog: 2p24 Cellular Component: centrosome; plasma membrane; spindle pole centrosome; cytosol; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; structural molecule activity; ATP binding Biological Process: regulation of cell adhesion; axon guidance; dendrite morphogenesis; rhythmic process; cytokinesis; regulation of circadian rhythm; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; neural tube closure; regulation of focal adhesion formation; ephrin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; centrosome duplication; regulation of keratinocyte differentiation |
| NCBI Summary: | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75116 |
| NCBI GenInfo Identifier: | 269849761 |
| NCBI Gene ID: | 9475 |
| NCBI Accession: | O75116.4 |
| UniProt Related Accession: | O75116 |
| Molecular Weight: | |
| NCBI Full Name: | Rho-associated protein kinase 2 |
| NCBI Synonym Full Names: | Rho-associated, coiled-coil containing protein kinase 2 |
| NCBI Official Symbol: | ROCK2 |
| NCBI Official Synonym Symbols: | ROCK-II |
| NCBI Protein Information: | rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II |
| UniProt Protein Name: | Rho-associated protein kinase 2 |
| UniProt Synonym Protein Names: | Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2 |
| Protein Family: | Rho-associated protein kinase |
| UniProt Gene Name: | ROCK2 |
 | Western blot analysis of extracts of various cell lines, using ROCK2 antibody (CAB5698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat brain using ROCK2 Rabbit pAb (CAB5698) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using ROCK2 Rabbit pAb (CAB5698) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using ROCK2 Rabbit pAb (CAB5698) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ROCK2 antibody (CAB5698). Blue: DAPI for nuclear staining. |
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