Anti-RNF185 Antibody (CAB16158)
- SKU:
- CAB16158
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-RNF185 Antibody |
| Antibody SKU: | CAB16158 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RNF185 (NP_689480.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RNF185 (NP_689480.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASK GPSA SASP ENSS AGGP SGSS NGAG ESGG QDST FECN ICLD TAKD AVIS LCGH LFCW PCLH QWLE TRPN RQVC PVCK AGIS RDKV IPLY GRGS TGQQ DPRE KTPP RPQG QRPE PENR GGFQ GFGF GD |
| Gene ID: | 91445 |
| Uniprot: | Q96GF1 |
| Cellular Location: | Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein |
| Calculated MW: | 14kDa/20kDa |
| Observed MW: | 20kDa |
| Synonyms: | RNF185 |
| Background: |
| UniProt Protein Function: | RNF185: Mitochondrial E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: endoplasmic reticulum Molecular Function:protein binding Biological Process: ER-associated protein catabolic process; protein autoubiquitination |
| UniProt Code: | Q96GF1 |
| NCBI GenInfo Identifier: | 74751883 |
| NCBI Gene ID: | 91445 |
| NCBI Accession: | Q96GF1.1 |
| UniProt Secondary Accession: | Q96GF1,Q8N900, A8K5C1, A9X3T8, |
| UniProt Related Accession: | Q96GF1 |
| Molecular Weight: | 14,139 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF185 |
| NCBI Synonym Full Names: | ring finger protein 185 |
| NCBI Official Symbol: | RNF185 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF185 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF185 |
| UniProt Synonym Protein Names: | RING finger protein 185 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF185 |
| UniProt Entry Name: | RN185_HUMAN |
 | Western blot analysis of extracts of Mouse testis, using RNF185 Rabbit pAb (CAB16158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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