Anti-RNF10 Antibody (CAB12296)
- SKU:
- CAB12296
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-RNF10 Antibody |
| Antibody SKU: | CAB12296 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 552-811 of human RNF10 (NP_055683.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, Mouse heart, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 552-811 of human RNF10 (NP_055683.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SMSE DVRQ RHRY LSHL PLTC EFSI CELA LQPP VVSK ETLE MFSD DIEK RKRQ RQKK AREE RRRE RRIE IEEN KKQG KYPE VHIP LENL QQFP AFNS YTCS SDSA LGPT STEG HGAL SISP LSRS PGSH ADFL LTPL SPTA SQGS PSFC VGSL EEDS PFPS FAQM LRVG KAKA DVWP KTAP KKDE NSLV PPAP VDSD GESD NSDR VPVP SFQN SFSQ AIEA AFMK LDTP ATSD PLSE EKGG KKRK KQKQ KLLF STSV VHTK |
| Gene ID: | 9921 |
| Uniprot: | Q8N5U6 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 89kDa/90kDa |
| Observed MW: | 100kDa |
| Synonyms: | RNF10, RIE2 |
| Background: | The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known. |
| UniProt Protein Function: | RNF10: Transcriptional factor involved in the regulation of MAG expression. Participates in the peripheral nerve development and Schawnn cell differentiation. Belongs to the RNF10 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: positive regulation of myelination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein autoubiquitination |
| NCBI Summary: | The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N5U6 |
| NCBI GenInfo Identifier: | 117949776 |
| NCBI Gene ID: | 9921 |
| NCBI Accession: | Q8N5U6.2 |
| UniProt Secondary Accession: | Q8N5U6,Q92550, Q9NPP8, Q9ULW4, |
| UniProt Related Accession: | Q8N5U6 |
| Molecular Weight: | 90,497 Da |
| NCBI Full Name: | RING finger protein 10 |
| NCBI Synonym Full Names: | ring finger protein 10 |
| NCBI Official Symbol: | RNF10 |
| NCBI Official Synonym Symbols: | RIE2 |
| NCBI Protein Information: | RING finger protein 10 |
| UniProt Protein Name: | RING finger protein 10 |
| Protein Family: | RING finger protein |
| UniProt Gene Name: | RNF10 |
| UniProt Entry Name: | RNF10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNF10 antibody (CAB12296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using RNF10 antibody (CAB12296) at dilution of 1:100 (40x lens). |
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