Anti-RNASEL Antibody (CAB9840)
- SKU:
- CAB9840
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-RNASEL Antibody |
| Antibody SKU: | CAB9840 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HT-29, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 472-741 of human RNASEL (NP_066956.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VQEL HLSC GYTH QDLQ PQNI LIDS KKAA HLAD FDKS IKWA GDPQ EVKR DLED LGRL VLYV VKKG SISF EDLK AQSN EEVV QLSP DEET KDLI HRLF HPGE HVRD CLSD LLGH PFFW TWES RYRT LRNV GNES DIKT RKSE SEIL RLLQ PGPS EHSK SFDK WTTK INEC VMKK MNKF YEKR GNFY QNTV GDLL KFIR NLGE HIDE EKHK KMKL KIGD PSLY FQKT FPDL VIYV YTKL QNTE YRKH FPQT HSPN KPQC DGAG GASG LASP GC |
| Gene ID: | 6041 |
| Uniprot: | Q05823 |
| Cellular Location: | Cytoplasm, Mitochondrion |
| Calculated MW: | 73kDa/83kDa |
| Observed MW: | 84kDa |
| Synonyms: | RNASEL, PRCA1, RNS4 |
| Background: | This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. |
| UniProt Protein Function: | Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length. |
| NCBI Summary: | This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q05823 |
| NCBI GenInfo Identifier: | 10863929 |
| NCBI Gene ID: | 6041 |
| NCBI Accession: | NP_066956.1 |
| UniProt Secondary Accession: | Q05823,Q5W0L2, Q6AI46, |
| UniProt Related Accession: | Q05823 |
| Molecular Weight: | 73,416 Da |
| NCBI Full Name: | 2-5A-dependent ribonuclease |
| NCBI Synonym Full Names: | ribonuclease L |
| NCBI Official Symbol: | RNASEL |
| NCBI Official Synonym Symbols: | RNS4; PRCA1 |
| NCBI Protein Information: | 2-5A-dependent ribonuclease |
| UniProt Protein Name: | 2-5A-dependent ribonuclease |
| UniProt Synonym Protein Names: | Ribonuclease 4; Ribonuclease L; RNase L |
| Protein Family: | 2-5A-dependent ribonuclease |
| UniProt Gene Name: | RNASEL |
 | Western blot analysis of extracts of various cell lines, using RNASEL antibody (CAB9840) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RNASEL antibody (CAB9840) at dilution of 1:100 (40x lens). |
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