Anti-RhoA Antibody (CAB0272)

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SKU:
CAB0272
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-RhoA Antibody
Antibody SKU:CAB0272
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RhoA (NP_001655.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse spleen, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RhoA (NP_001655.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TPEV KHFC PNVP IILV GNKK DLRN DEHT RREL AKMK QEPV KPEE GRDM ANRI GAFG YMEC SAKT KDGV REVF EMAT RAAL QARR GKKK SGCL VL
Gene ID:387
Uniprot:P61586
Cellular Location:Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Cytoplasmic side, Lipid-anchor, Midbody, cell cortex, cytoskeleton, lamellipodium
Calculated MW:21kDa
Observed MW:22kDa
Synonyms:ARH12, ARHA, RHO12, RHOH12, Rho, RHOA, RhoA
Background:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
UniProt Protein Function:RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
UniProt Protein Details:

Protein type:G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function:GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation

NCBI Summary:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
UniProt Code:P61586
NCBI GenInfo Identifier:47606458
NCBI Gene ID:387
NCBI Accession:P61586.1
UniProt Related Accession:P61586
Molecular Weight:22kDa
NCBI Full Name:Transforming protein RhoA
NCBI Synonym Full Names:ras homolog family member A
NCBI Official Symbol:RHOA  
NCBI Official Synonym Symbols:ARHA; ARH12; RHO12; RHOH12  
NCBI Protein Information:transforming protein RhoA
UniProt Protein Name:Transforming protein RhoA
UniProt Synonym Protein Names:Rho cDNA clone 12; h12
Protein Family:Rhotekin
UniProt Gene Name:RHOA  
UniProt Entry Name:RHOA_HUMAN
Anti-RhoA Antibody (CAB0272)Western blot analysis of extracts of various cell lines, using RhoA antibody (CAB0272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RhoA Antibody (CAB0272)Immunofluorescence analysis of NIH-3T3 cells using RhoA Rabbit pAb (CAB0272) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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