Anti-RHD 항체 (CAB15092)

Antibody Name:	Anti-RHD Antibody
Antibody SKU:	CAB15092
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RHD (NP_001121163.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	mouse liver, rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human RHD (NP_001121163.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LGNL RMVI SNIF NTDY HMNM MHIY VFAA YFGL SVAW CLPK PLPE GTED KDQT ATIP SLSA MLGA LFLW MFWP SFNS ALLR SPIE RKNA VFNT YYAV AVSV V
Gene ID:	6007
Uniprot:	Q02161
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	34kDa/41kDa/43kDa/45kDa/53kDa
Observed MW:	36kDa

Synonyms:

RHD, CD240D, DIIIc, RH, RH30, RHCED, RHDVA(TT), RHDel, RHPII, RHXIII, Rh4, RhDCw, RhII, RhK562-II, RhPI

Background:

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RHD: May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: integral to plasma membrane Molecular Function:ammonium transmembrane transporter activity Biological Process: nitrogen utilization; organic cation transport
NCBI Summary:	The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q02161
NCBI GenInfo Identifier:	296452980
NCBI Gene ID:	6007
NCBI Accession:	Q02161.3
UniProt Secondary Accession:	Q02161,Q02162, Q07618, Q16147, Q16235, Q16355, Q5VSK0 Q5XLS9, Q5XLT1, Q5XLT2, Q9NPK0, Q9UQ20,
UniProt Related Accession:	Q02161
Molecular Weight:	41,694 Da
NCBI Full Name:	Blood group Rh(D) polypeptide
NCBI Synonym Full Names:	Rh blood group D antigen
NCBI Official Symbol:	RHD
NCBI Official Synonym Symbols:	RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
NCBI Protein Information:	blood group Rh(D) polypeptide
UniProt Protein Name:	Blood group Rh(D) polypeptide
UniProt Synonym Protein Names:	RHXIII; Rh polypeptide 2; RhPII; Rhesus D antigen; CD_antigen: CD240D
UniProt Gene Name:	RHD
UniProt Entry Name:	RHD_HUMAN

Western blot analysis of extracts of various cell lines, using RHD antibody (CAB15092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

Anti-RHD Antibody (CAB15091)

RHD Antibody (PACO06910)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)