Anti-RFFL Antibody (CAB6489)

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SKU:
CAB6489
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-RFFL Antibody
Antibody SKU:CAB6489
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_001017368.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_001017368.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWAT CCNW FCLD GQPE EVPP PQGA RMQA YSNP GYSS FPSP TGLE PSCK SCGA HFAN TARK QTCL DCKK NFCM TCSS QVGN GPRL CLLC QRFR ATAF QREE LMKM KVKD LRDY LSLH DIST EMCR EKEE LVLL VLGQ QPVI SQED RTRA STLS PDFP EQQA FLTQ PHSS MVPP TSPN LPSS SAQA TSVP PA
Gene ID:117584
Uniprot:Q8WZ73
Cellular Location:Cell membrane, Cytoplasm, Peripheral membrane protein, Recycling endosome membrane, cytosol
Calculated MW:36kDa/39kDa/40kDa
Observed MW:50kDa
Synonyms:RFFL, CARP-2, CARP2, FRING, RIFIFYLIN, RNF189, RNF34L
Background:
UniProt Protein Function:RFFL: Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi membrane; membrane; recycling endosome membrane; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; endosome membrane; cytosol

Molecular Function:protein binding; protease binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; protein kinase binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; intracellular protein transport; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of TOR signaling pathway

UniProt Code:Q8WZ73
NCBI GenInfo Identifier:74760639
NCBI Gene ID:117584
NCBI Accession:Q8WZ73.1
UniProt Secondary Accession:Q8WZ73,Q8NHW0, Q8TBY7, Q96BE6, E1P633,
UniProt Related Accession:Q8WZ73
Molecular Weight:363
NCBI Full Name:E3 ubiquitin-protein ligase rififylin
NCBI Synonym Full Names:ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
NCBI Official Symbol:RFFL  
NCBI Official Synonym Symbols:CARP2; FRING; CARP-2; RNF189; RNF34L; RIFIFYLIN  
NCBI Protein Information:E3 ubiquitin-protein ligase rififylin; RING finger protein 189; caspase regulator CARP2; FYVE-RING finger protein SAKURA; ring finger and FYVE-like domain containing 1; caspases-8 and -10-associated RING finger protein 2; RING finger and FYVE-like domain-containing protein 1
UniProt Protein Name:E3 ubiquitin-protein ligase rififylin
UniProt Synonym Protein Names:Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; CARP-2; FYVE-RING finger protein Sakura; Fring; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RFFL  
UniProt Entry Name:RFFL_HUMAN
Anti-RFFL Antibody (CAB6489)Western blot analysis of extracts of U-251MG cells, using RFFL antibody (CAB6489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.

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