Anti-RECK 항체 (CAB6718)

Antibody Name:	Anti-RECK Antibody
Antibody SKU:	CAB6718
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-210 of human RECK (NP_066934.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF-7, H460, Mouse lung, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-210 of human RECK (NP_066934.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALEC RQAC KQAS SKND ISKV CRKE YENA LFSC ISRN EMGS VCCS YAGH HTNC REYC QAIF RTDS SPGP SQIK AVEN YCAS ISPQ LIHC VNNY TQSY PMRN P
Gene ID:	8434
Uniprot:	O95980
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	106kDa
Observed MW:	106kDa

Synonyms:

RECK, ST15

Background:

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RECK: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. Interacts with MMP-9. Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
UniProt Protein Details:	Protein type:Membrane protein, GPI anchor; Tumor suppressor Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: membrane; plasma membrane Molecular Function:serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity Biological Process: extracellular matrix organization and biogenesis; blood vessel maturation; embryo implantation
NCBI Summary:	The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. [provided by RefSeq, Jul 2008]
UniProt Code:	O95980
NCBI GenInfo Identifier:	20178043
NCBI Gene ID:	8434
NCBI Accession:	O95980.1
UniProt Secondary Accession:	O95980,Q5W0K6, Q8WX37, B2RNS1,
UniProt Related Accession:	O95980
Molecular Weight:	971
NCBI Full Name:	Reversion-inducing cysteine-rich protein with Kazal motifs
NCBI Synonym Full Names:	reversion-inducing-cysteine-rich protein with kazal motifs
NCBI Official Symbol:	RECK
NCBI Official Synonym Symbols:	ST15
NCBI Protein Information:	reversion-inducing cysteine-rich protein with Kazal motifs; suppressor of tumorigenicity 15 protein; membrane-anchored glycoprotein (metastasis and invasion); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs); suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs)
UniProt Protein Name:	Reversion-inducing cysteine-rich protein with Kazal motifs
UniProt Synonym Protein Names:	Suppressor of tumorigenicity 15 protein
Protein Family:	Reversion-inducing cysteine-rich protein
UniProt Gene Name:	RECK
UniProt Entry Name:	RECK_HUMAN

	Western blot analysis of extracts of various cell lines, using RECK antibody (CAB6718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using RECK Polyclonal Antibody (CAB6718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
RECK Antibody (PACO05513)	Human RECK ELISA Kit
RECK Antibody (PACO11791)	Human Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)