Anti-RBM25 Antibody (CAB15496)
- SKU:
- CAB15496
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-RBM25 Antibody |
| Antibody SKU: | CAB15496 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | DU145, OVCAR3, HepG2, Jurkat, NIH/3T3, mouse brain, mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KPTL RPIS SAPS VSSA SGNA TPNT PGDE SPCG IIIP HENS PDQQ QPEE HRPK IGLS LKLG ASNS PGQP NSVK RKKL PVDS VFNK FEDE DSDD VPRK RKLV PLDY GEDD KNAT KGTV NTEE KRKH IKSL IEKI PTAK PELF AYPL DWSI VDSI LMER RIRP WINK KIIE YIGE EEAT LVDF VCSK VMAH SSPQ SILD DVAM VLDE EAEV FIVK MWRL LIYE TEAK KIGL VK |
| Gene ID: | 58517 |
| Uniprot: | P49756 |
| Cellular Location: | Cytoplasm, Nucleus speckle |
| Calculated MW: | 17kDa/32kDa/33kDa/100kDa |
| Observed MW: | 120kDa |
| Synonyms: | RBM25, NET52, RED120, RNPC7, S164, Snu71, fSAP94 |
| Background: |
| UniProt Code: | P49756 |
| NCBI GenInfo Identifier: | 55741709 |
| NCBI Gene ID: | 58517 |
| NCBI Accession: | NP_067062.1 |
| UniProt Secondary Accession: | P49756,Q2TA72, Q5XJ17, Q6P665, Q9H6A1, Q9UEQ5, Q9UIE9 A0PJL9, B2RNA8, B3KT03, |
| UniProt Related Accession: | P49756,Q16083 |
| Molecular Weight: | 100,185 Da |
| NCBI Full Name: | RNA-binding protein 25 |
| NCBI Synonym Full Names: | RNA binding motif protein 25 |
| NCBI Official Symbol: | RBM25 |
| NCBI Official Synonym Symbols: | S164; NET52; RNPC7; Snu71; RED120; fSAP94; MGC105088; MGC117168 |
| NCBI Protein Information: | RNA-binding protein 25; OTTHUMP00000236132; OTTHUMP00000236134; RNA-binding motif protein 25; Arg/Glu/Asp-rich protein, 120 kDa; arg/Glu/Asp-rich protein of 120 kDa; RNA-binding region-containing protein 7; RNA-binding region (RNP1, RRM) containing 7; fun |
| UniProt Protein Name: | RNA-binding protein 25 |
| UniProt Synonym Protein Names: | Arg/Glu/Asp-rich protein of 120 kDa; RED120; Protein S164; RNA-binding motif protein 25; RNA-binding region-containing protein 7 |
| Protein Family: | RNA-binding protein |
| UniProt Gene Name: | RBM25 |
| UniProt Entry Name: | RBM25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBM25 antibody (CAB15496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of U-2 OS cells using RBM25 antibody (CAB15496) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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