Anti-RASSF5 Antibody (CAB7389)
- SKU:
- CAB7389
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-RASSF5 Antibody |
| Antibody SKU: | CAB7389 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, A-549, 293T, Jurkat, Mouse brain, Mouse liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human RASSF5 (NP_872606.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTVD SSMS SGYC SLDE ELED CFFT AKTT FFRN AQSK HLSK NVCK PVEE TQRP PTLQ EIKQ KIDS YNTR EKNC LGMK LSED GTYT GFIK VHLK LRRP VTVP AGIR PQSI YDAI KEVN LAAT TDKR TSFY LPLD AIKQ LHIS STTT VSEV IQGL LKKF MVVD NPQK FALF KRIH KDGQ VLFQ KLSI ADRP LYLR LLAG PDTE VLSF VLKE NETG EVEW DAFS IPEL QNFL TILE KEEQ DKIQ QVQK KYDK FRQK LEEA LRES QGKP G |
| Gene ID: | 83593 |
| Uniprot: | Q8WWW0 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 30kDa/37kDa/47kDa |
| Observed MW: | 37kDa, 45kDa |
| Synonyms: | RASSF5, Maxp1, NORE1, NORE1A, NORE1B, RAPL, RASSF3 |
| Background: | This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | RASSF5: Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS1 and KRAS induced apoptosis. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy; Cytoskeletal; Motility/polarity/chemotaxis; Tumor suppressor Chromosomal Location of Human Ortholog: 1q32.1 Molecular Function:protein binding |
| NCBI Summary: | This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WWW0 |
| NCBI GenInfo Identifier: | 74751587 |
| NCBI Gene ID: | 83593 |
| NCBI Accession: | Q8WWW0.1 |
| UniProt Secondary Accession: | Q8WWW0,Q5SY32, Q8WWV9, Q8WXF4, Q9BT99, A8K1E6, |
| UniProt Related Accession: | Q8WWW0 |
| Molecular Weight: | 47kDa |
| NCBI Full Name: | Ras association domain-containing protein 5 |
| NCBI Synonym Full Names: | Ras association domain family member 5 |
| NCBI Official Symbol: | RASSF5 |
| NCBI Official Synonym Symbols: | RAPL; Maxp1; NORE1; NORE1A; NORE1B; RASSF3 |
| NCBI Protein Information: | ras association domain-containing protein 5 |
| UniProt Protein Name: | Ras association domain-containing protein 5 |
| UniProt Synonym Protein Names: | New ras effector 1; Regulator for cell adhesion and polarization enriched in lymphoid tissues; RAPL |
| Protein Family: | Ras association domain-containing protein |
| UniProt Gene Name: | RASSF5 |
 | Western blot analysis of extracts of various cell lines, using RASSF5 antibody (CAB7389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human prostate using RASSF5 antibody (CAB7389) at dilution of 1:100 (40x lens). |
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