Anti-RARS Antibody (CAB6307)
- SKU:
- CAB6307
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-RARS Antibody |
| Antibody SKU: | CAB6307 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RARS (NP_002878.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HeLa, HepG2, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RARS (NP_002878.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDVL VSEC SARL LQQE EEIK SLTA EIDR LKNC GCLG ASPN LEQL QEEN LKLK YRLN ILRK SLQA ERNK PTKN MINI ISRL QEVF GHAI KAAY PDLE NPPL LVTP SQQA KFGD YQCN SAMG ISQM LKTK EQKV NPRE IAEN ITKH LPDN ECIE KVEI AGPG FINV HLRK DFVS EQLT SLLV NGVQ LPAL GENK KVIV DFSS PNIA KEMH VGHL RSTI IGES ISRL FEFA GYDV LRLN HVGD WGTQ FGML IAHL QDKF PDYL TVSP PIGD LQVF YKES KKRF DTEE EFKK RAYQ CVVL LQGK |
| Gene ID: | 5917 |
| Uniprot: | P54136 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 67kDa/75kDa |
| Observed MW: | 75kDa |
| Synonyms: | RARS, ArgRS, DALRD1, HLD9 |
| Background: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. |
| UniProt Protein Function: | RARS: Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Mitochondrial; RNA-binding; Translation; Ligase; EC 6.1.1.19 Chromosomal Location of Human Ortholog: 5q35.1 Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm Molecular Function:arginine-tRNA ligase activity; protein binding Biological Process: tRNA aminoacylation for protein translation Disease: Leukodystrophy, Hypomyelinating, 9 |
| NCBI Summary: | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P54136 |
| NCBI GenInfo Identifier: | 20178331 |
| NCBI Gene ID: | 5917 |
| NCBI Accession: | P54136.2 |
| UniProt Secondary Accession: | P54136,Q53GY4, Q9BWA1, B2RBS9, |
| UniProt Related Accession: | P54136 |
| Molecular Weight: | 67,140 Da |
| NCBI Full Name: | Arginine--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | arginyl-tRNA synthetase |
| NCBI Official Symbol: | RARS |
| NCBI Official Synonym Symbols: | HLD9; ArgRS; DALRD1 |
| NCBI Protein Information: | arginine--tRNA ligase, cytoplasmic |
| UniProt Protein Name: | Arginine--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Arginyl-tRNA synthetase; ArgRS |
| Protein Family: | Arginine--tRNA ligase |
| UniProt Gene Name: | RARS |
| UniProt Entry Name: | SYRC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RARS antibody (CAB6307) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RARS antibody (CAB6307) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using RARS antibody (CAB6307) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RARS antibody (CAB6307) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using RARS antibody (CAB6307). Blue: DAPI for nuclear staining. |
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