Anti-RAPGEF1 Antibody (CAB16050)
- SKU:
- CAB16050
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Frequently bought together:
Description
| Antibody Name: | Anti-RAPGEF1 Antibody |
| Antibody SKU: | CAB16050 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 844-1095 of human RAPGEF1 (NP_941372.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ARGV AARP GTLH DFHS HEIA EQLT LLDA ELFY KIEI PEVL LWAK EQNE EKSP NLTQ FTEH FNNM SYWV RSII MLQE KAQD RERL LLKF IKIM KHLR KLNN FNSY LAIL SALD SAPI RRLE WQKQ TSEG LAEY CTLI DSSS SFRA YRAA LSEV EPPC IPYL GLIL QDLT FVHL GNPD YIDG KVNF SKRW QQFN ILDS MRCF QQAH YDMR RNDD IINF FNDF SDHL AEEA LWEL SLKI KPRN ITRR KTDR EEKT |
| Gene ID: | 2889 |
| Uniprot: | Q13905 |
| Cellular Location: | Early endosome |
| Calculated MW: | 116kDa/120kDa/122kDa |
| Observed MW: | 120kDa |
| Synonyms: | RAPGEF1, C3G, GRF2 |
| Background: | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. |
| UniProt Protein Function: | RAPGEF1: an adaptor-type Src homology (SH)2-containing molecule. A guanine nucleotide releasing protein for Ras. Expressed ubiquitously in human adult and fetal tissues. Several alternatively spliced transcript variants have been described. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Ras; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: cytosol; early endosome; endosome Molecular Function:protein binding; Rap guanyl-nucleotide exchange factor activity Biological Process: activation of MAPKK activity; positive regulation of GTPase activity; Rap protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13905 |
| NCBI GenInfo Identifier: | 143811452 |
| NCBI Gene ID: | 2889 |
| NCBI Accession: | Q13905.3 |
| UniProt Secondary Accession: | Q13905,Q5JUE4, Q68DL3, Q8IV73, |
| UniProt Related Accession: | Q13905 |
| Molecular Weight: | 122,287 Da |
| NCBI Full Name: | Rap guanine nucleotide exchange factor 1 |
| NCBI Synonym Full Names: | Rap guanine nucleotide exchange factor 1 |
| NCBI Official Symbol: | RAPGEF1 |
| NCBI Official Synonym Symbols: | C3G; GRF2 |
| NCBI Protein Information: | rap guanine nucleotide exchange factor 1 |
| UniProt Protein Name: | Rap guanine nucleotide exchange factor 1 |
| UniProt Synonym Protein Names: | CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; Protein C3G |
| Protein Family: | Rap guanine nucleotide exchange factor |
| UniProt Gene Name: | RAPGEF1 |
| UniProt Entry Name: | RPGF1_HUMAN |
 | Western blot analysis of extracts of Mouse liver, using RAPGEF1 Rabbit pAb (CAB16050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using RAPGEF1 Polyclonal Antibody (CAB16050) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RAPGEF1 Polyclonal Antibody (CAB16050) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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