Anti-RANGAP1 Antibody (CAB13347)
- SKU:
- CAB13347
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-RANGAP1 Antibody |
| Antibody SKU: | CAB13347 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RANGAP1 (NP_002874.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | BT-474, HeLa, MCF7, SH-SY5Y, A-549, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RANGAP1 (NP_002874.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PQQR GQGE KSAT PSRK ILDP NTGE PAPV LSSP PPAD VSTF LAFP SPEK LLRL GPKS SVLI AQQT DTSD PEKV VSAF LKVS SVFK DEAT VRMA VQDA VDAL MQKA FNSS SFNS NTFL TRLL VHMG LLKS EDKV KAIA NLYG PLMA LNHM VQQD YFPK ALAP LLLA FVTK PNSA LESC SFAR HSLL QTLY KV |
| Gene ID: | 5905 |
| Uniprot: | P46060 |
| Cellular Location: | Chromosome, Cytoplasm, Cytoplasmic side, Nucleus membrane, Peripheral membrane protein, centromere, cytoskeleton, kinetochore, spindle pole |
| Calculated MW: | 63kDa |
| Observed MW: | 64/90kDa |
| Synonyms: | RANGAP1, Fug1, RANGAP, SD |
| Background: | This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RANGAP1: a cytoplasmic GTPase activator for the Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. Homodimerizes. Forms a tight complex in association with RANBP2 and the ubiquitin-conjugating enzyme E2 (UBC9). |
| UniProt Protein Details: | Protein type:GAPs; GAPs, Ras Chromosomal Location of Human Ortholog: 22q13 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; kinetochore; nuclear envelope; nuclear membrane; nuclear pore Molecular Function:protein binding Biological Process: negative regulation of protein export from nucleus; protein sumoylation; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P46060 |
| NCBI GenInfo Identifier: | 1172922 |
| NCBI Gene ID: | 5905 |
| NCBI Accession: | P46060.1 |
| UniProt Secondary Accession: | P46060,Q96JJ2, |
| UniProt Related Accession: | P46060 |
| Molecular Weight: | 63,542 Da |
| NCBI Full Name: | Ran GTPase-activating protein 1 |
| NCBI Synonym Full Names: | Ran GTPase activating protein 1 |
| NCBI Official Symbol: | RANGAP1 |
| NCBI Official Synonym Symbols: | SD; Fug1; RANGAP |
| NCBI Protein Information: | ran GTPase-activating protein 1 |
| UniProt Protein Name: | Ran GTPase-activating protein 1 |
| Protein Family: | RAN GTPase-activating protein |
| UniProt Gene Name: | RANGAP1 |
| UniProt Entry Name: | RAGP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RANGAP1 antibody (CAB13347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using RANGAP1 Rabbit pAb (CAB13347) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using RANGAP1 Rabbit pAb (CAB13347) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using RANGAP1 antibody (CAB13347). |
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