Anti-RAC3 Antibody (CAB7498)

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SKU:
CAB7498
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-RAC3 Antibody
Antibody SKU:CAB7498
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC3 (NP_005043.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BT-474, HL-60, Human placenta, Mouse lung, Mouse brain, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC3 (NP_005043.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQAI KCVV VGDG AVGK TCLL ISYT TNAF PGEY IPTV FDNY SANV MVDG KPVN LGLW DTAG QEDY DRLR PLSY PQTD VFLI CFSL VSPA SFEN VRAK WYPE VRHH CPHT PILL VGTK LDLR DDKD TIER LRDK KLAP ITYP QGLA MARE IGSV KYLE CSAL TQRG LKTV FDEA IRAV LCPP PVKK PGKK CTVF
Gene ID:5881
Uniprot:P60763
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Endomembrane system, cytoskeleton, lamellipodium, perinuclear region
Calculated MW:21kDa
Observed MW:21kDa
Synonyms:RAC3
Background:The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:RAC3: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation. Highest levels in brain, also detected in heart, placenta and pancreas. Belongs to the small GTPase superfamily. Rho family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: filamentous actin; neuron projection; growth cone; cell soma; perinuclear region of cytoplasm; lamellipodium; plasma membrane; endomembrane system; cytosol

Molecular Function:GTPase activity; protein binding; GTP binding; calcium-dependent protein binding

Biological Process: regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; positive regulation of cell adhesion mediated by integrin; cell projection biogenesis; actin cytoskeleton organization and biogenesis; neuromuscular process controlling balance; neurite development

NCBI Summary:The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:P60763
NCBI GenInfo Identifier:46397673
NCBI Gene ID:5881
NCBI Accession:P60763.1
UniProt Related Accession:P60763
Molecular Weight:
NCBI Full Name:Ras-related C3 botulinum toxin substrate 3
NCBI Synonym Full Names:Rac family small GTPase 3
NCBI Official Symbol:RAC3  
NCBI Protein Information:ras-related C3 botulinum toxin substrate 3
UniProt Protein Name:Ras-related C3 botulinum toxin substrate 3
UniProt Synonym Protein Names:p21-Rac3
Protein Family:Rac-like GTP-binding protein
UniProt Gene Name:RAC3  
UniProt Entry Name:RAC3_HUMAN
Anti-RAC3 Antibody (CAB7498)Western blot analysis of extracts of various cell lines, using RAC3 antibody (CAB7498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-RAC3 Antibody (CAB7498)Immunofluorescence analysis of A549 cells using RAC3 antibody (CAB7498). Blue: DAPI for nuclear staining.

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