Anti-R-spondin-3 Antibody (CAB8389)
- SKU:
- CAB8389
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-R-spondin-3 Antibody |
| Antibody SKU: | CAB8389 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RSPO3 (NP_116173.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, NCI-H460, HeLa, Mouse liver, Mouse skin, Mouse brain, Mouse spinal cord, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RSPO3 (NP_116173.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHLR LISW LFII LNFM EYIG SQNA SRGR RQRR MHPN VSQG CQGG CATC SDYN GCLS CKPR LFFA LERI GMKQ IGVC LSSC PSGY YGTR YPDI NKCT KCKA |
| Gene ID: | 84870 |
| Uniprot: | Q9BXY4 |
| Cellular Location: | Secreted |
| Calculated MW: | 30kDa/33kDa |
| Observed MW: | 39kDa |
| Synonyms: | RSPO3, CRISTIN1, PWTSR, THSD2 |
| Background: | This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. |
| UniProt Protein Function: | R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6q22.33 Cellular Component: extracellular space; extracellular region Molecular Function:heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding Biological Process: Wnt receptor signaling pathway through beta-catenin |
| NCBI Summary: | This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BXY4 |
| NCBI GenInfo Identifier: | 74752442 |
| NCBI Gene ID: | 84870 |
| NCBI Accession: | Q9BXY4.1 |
| UniProt Secondary Accession: | Q9BXY4,Q5VTV4, Q96K87, B2RC27, |
| UniProt Related Accession: | Q9BXY4 |
| Molecular Weight: | 33,233 Da |
| NCBI Full Name: | R-spondin-3 |
| NCBI Synonym Full Names: | R-spondin 3 |
| NCBI Official Symbol: | RSPO3 |
| NCBI Official Synonym Symbols: | PWTSR; THSD2; CRISTIN1 |
| NCBI Protein Information: | R-spondin-3; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2 |
| UniProt Protein Name: | R-spondin-3 |
| UniProt Synonym Protein Names: | Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2 |
| Protein Family: | R-spondin |
| UniProt Gene Name: | RSPO3 |
| UniProt Entry Name: | RSPO3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RSPO3 antibody (CAB8389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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