Anti-PYGM Antibody (CAB9392)

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SKU:
CAB9392
Product type:
Antibody
Application:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-PYGM Antibody
Antibody SKU:CAB9392
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PYGM (NP_005600.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse skeletal muscle, Mouse brain, Mouse lung, Rat skeletal muscle
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PYGM (NP_005600.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEE AGEE NFFI FGMR VEDV DKLD QRGY NAQE YYDR IPEL RQVI EQLS SGFF SPKQ PDLF KDIV NMLM HHDR FKVF ADYE DYIK CQEK VSAL YKNP REWT RMVI RNIA TSGK FSSD RTIA QYAR EIWG VEPS RQRL PAPD EAI
Gene ID:5837
Uniprot:P11217
Cellular Location:
Calculated MW:87kDa/97kDa
Observed MW:100kDa
Synonyms:PYGM
Background:This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.
UniProt Protein Details:

Protein type:Transferase; Phosphorylase; Endoplasmic reticulum; Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytoplasm; cytosol

Molecular Function:protein binding; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; glycogen metabolic process

Disease: Glycogen Storage Disease V

NCBI Summary:This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]
UniProt Code:P11217
NCBI GenInfo Identifier:3041717
NCBI Gene ID:5837
NCBI Accession:P11217.6
UniProt Secondary Accession:P11217,A0AVK1, A6NDY6,
UniProt Related Accession:P11217
Molecular Weight:87,317 Da
NCBI Full Name:Glycogen phosphorylase, muscle form
NCBI Synonym Full Names:phosphorylase, glycogen, muscle
NCBI Official Symbol:PYGM  
NCBI Protein Information:glycogen phosphorylase, muscle form
UniProt Protein Name:Glycogen phosphorylase, muscle form
UniProt Synonym Protein Names:Myophosphorylase
Protein Family:Glycogen phosphorylase
UniProt Gene Name:PYGM  
UniProt Entry Name:PYGM_HUMAN
Anti-PYGM Polyclonal Antibody (CAB9392)Western blot analysis of extracts of various cell lines, using PYGM antibody (CAB9392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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