Anti-PYGB Antibody (CAB19910)[KO Validated]

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SKU:
CAB19910
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-PYGB Antibody [KO Validated]
Antibody SKU:CAB19910
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PYGB.
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:20 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human PYGB.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5834
Uniprot:P11216
Cellular Location:
Calculated MW:96kDa
Observed MW:97KDa
Anti-PYGB Antibody [KO Validated] (CAB19910)Western blot analysis of extracts from normal (control) and PYGB knockout (KO) HeLa cells, using PYGB antibody (CAB19910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PYGB, GPBB
Background:The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation.
UniProt Protein Function:PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.
UniProt Protein Details:

Protein type:Phosphorylase; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Transferase; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cytoplasm; membrane

Molecular Function:glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding

Biological Process: glycogen catabolic process

NCBI Summary:The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]
UniProt Code:P11216
NCBI GenInfo Identifier:20178317
NCBI Gene ID:5834
NCBI Accession:P11216.5
UniProt Secondary Accession:P11216,Q96AK1, Q9NPX8,
UniProt Related Accession:P11216
Molecular Weight:96,696 Da
NCBI Full Name:Glycogen phosphorylase, brain form
NCBI Synonym Full Names:phosphorylase, glycogen; brain
NCBI Official Symbol:PYGB  
NCBI Official Synonym Symbols:GPBB  
NCBI Protein Information:glycogen phosphorylase, brain form
UniProt Protein Name:Glycogen phosphorylase, brain form
Protein Family:Glycogen phosphorylase
UniProt Gene Name:PYGB  
UniProt Entry Name:PYGB_HUMAN

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