Anti-PYCR1 Antibody (CAB13346)
- SKU:
- CAB13346
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-PYCR1 Antibody |
| Antibody SKU: | CAB13346 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, HepG2, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSVG FIGA GQLA FALA KGFT AAGV LAAH KIMA SSPD MDLA TVSA LRKM GVKL TPHN KETV QHSD VLFL AVKP HIIP FILD EIGA DIED RHIV VSCA AGVT ISSI EKKL SAFR PAPR VIRC MTNT PVVV REGA TVYA TGTH AQVE DGRL MEQL LSSV GFCT EVEE DLID AVTG LSGS GPAY AFTA LDAL ADGG VKMG LPRR LAVR LGAQ ALLG AAKM LLHS EQHP GQLK DNVS SPGG ATIH ALHV LESG GFRS LLIN AVEA SCIR TREL QSMA DQEQ VSPA AIKK TILD KVKL DSPA GTAL SPSG HTKL LPRS LAPA GKD |
| Gene ID: | 5831 |
| Uniprot: | P32322 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 33kDa/35kDa |
| Observed MW: | 35kDa |
| Synonyms: | PYCR1, ARCL2B, ARCL3B, P5C, P5CR, PIG45, PP222, PRO3, PYCR |
| Background: | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family. |
| UniProt Protein Details: | Protein type:EC 1.5.1.2; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity Biological Process: amino acid biosynthetic process; proline biosynthetic process; regulation of mitochondrial membrane potential Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib |
| NCBI Summary: | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P32322 |
| NCBI GenInfo Identifier: | 60416434 |
| NCBI Gene ID: | 5831 |
| NCBI Accession: | P32322.2 |
| UniProt Secondary Accession: | P32322,Q6FHI4, Q96DI6, Q9HBQ4, A6NFM2, B4DMU0, |
| UniProt Related Accession: | P32322 |
| Molecular Weight: | 35,981 Da |
| NCBI Full Name: | Pyrroline-5-carboxylate reductase 1, mitochondrial |
| NCBI Synonym Full Names: | pyrroline-5-carboxylate reductase 1 |
| NCBI Official Symbol: | PYCR1 |
| NCBI Official Synonym Symbols: | P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B |
| NCBI Protein Information: | pyrroline-5-carboxylate reductase 1, mitochondrial |
| UniProt Protein Name: | Pyrroline-5-carboxylate reductase 1, mitochondrial |
| Protein Family: | Pyrroline-5-carboxylate reductase |
| UniProt Gene Name: | PYCR1 |
| UniProt Entry Name: | P5CR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PYCR1 antibody (CAB13346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat liver using PYCR1 antibody (CAB13346) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using PYCR1 antibody (CAB13346) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using PYCR1 antibody (CAB13346) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using PYCR1 antibody (CAB13346) at dilution of 1:200 (40x lens). |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
