Anti-PTH1R Antibody (CAB1744)
- SKU:
- CAB1744
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PTH1R Antibody |
| Antibody SKU: | CAB1744 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 464-593 of human PTH1R (NP_000307.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, MCF7, U-251MG, DU145, THP-1, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 464-593 of human PTH1R (NP_000307.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GEVQ AEIK KSWS RWTL ALDF KRKA RSGS SSYS YGPM VSHT SVTN VGPR VGLG LPLS PRLL PTAT TNGH PQLP GHAK PGTP ALET LETT PPAM AAPK DDGF LNGS CSGL DEEA SGPE RPPA LLQE EWET VM |
| Gene ID: | 5745 |
| Uniprot: | Q03431 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 66kDa |
| Observed MW: | 66kDa |
| Synonyms: | PTH1R, PFE, PTHR, PTHR1 |
| Background: | The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | PTHR: This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in PTH1R are the cause of Jansen metaphyseal chondrodysplasia (JMC). JMC is a rare autosomal dominant disorder characterized by a short-limbed dwarfism associated with hypercalcemia and normal or low serum concentrations of the two parathyroid hormones. Defects in PTH1R are the cause of chondrodysplasia Blomstrand type (BOCD). BOCD is a severe skeletal dysplasia. Defects in PTH1R may be a cause of enchondromatosis multiple (ENCHOM). Enchondromas are common benign cartilage tumors of bone. They can occur as solitary lesions or as multiple lesions in enchondromatosis (Ollier and Maffucci diseases). Clinical problems caused by enchondromas include skeletal deformity and the potential for malignant change to osteosarcoma. Defects in PTH1R are the cause of Eiken skeletal dysplasia (EISD); also known as bone modeling defect of hands and feet. It is a rare familial autosomal recessive skeletal dysplasia. It is characterized by multiple epiphyseal dysplasia, with extremely retarded ossification, principally of the epiphyses, pelvis, hands and feet, as well as by abnormal modeling of the bones in hands and feet, abnormal persistence of cartilage in the pelvis and mild growth retardation. Defects in PTH1R are a cause of primary failure of tooth eruption (PFE). PFE is a rare condition that has high penetrance and variable expressivity and in which tooth retention occurs without evidence of any obvious mechanical interference. Instead, malfunction of the eruptive mechanism itself appears to cause nonankylosed permanent teeth to fail to erupt, although the eruption pathway has been cleared by bone resorption. Belongs to the G-protein coupled receptor 2 family. |
| UniProt Protein Details: | Protein type:GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR Chromosomal Location of Human Ortholog: 3p22-p21.1 Cellular Component: basolateral plasma membrane; integral to plasma membrane; brush border membrane; apical plasma membrane; cytoplasm; plasma membrane; nucleus; receptor complex Molecular Function:protein binding; protein self-association; peptide hormone binding; parathyroid hormone receptor activity Biological Process: cell maturation; G-protein signaling, adenylate cyclase activating pathway; bone mineralization; osteoblast development; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of cell proliferation; chondrocyte differentiation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); skeletal development; bone resorption; aging Disease: Eiken Syndrome; Chondrodysplasia, Blomstrand Type; Failure Of Tooth Eruption, Primary; Metaphyseal Chondrodysplasia, Jansen Type |
| NCBI Summary: | The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | Q03431 |
| NCBI GenInfo Identifier: | 417555 |
| NCBI Gene ID: | 5745 |
| NCBI Accession: | Q03431.1 |
| UniProt Secondary Accession: | Q03431,Q2M1U3, |
| UniProt Related Accession: | Q03431 |
| Molecular Weight: | 66,361 Da |
| NCBI Full Name: | Parathyroid hormone/parathyroid hormone-related peptide receptor |
| NCBI Synonym Full Names: | parathyroid hormone 1 receptor |
| NCBI Official Symbol: | PTH1R |
| NCBI Official Synonym Symbols: | PFE; PTHR; PTHR1 |
| NCBI Protein Information: | parathyroid hormone/parathyroid hormone-related peptide receptor; PTH1 receptor; PTH/PTHr receptor; PTH/PTHrP type I receptor; parathyroid hormone receptor 1; seven transmembrane helix receptor; parathyroid hormone/parathyroid hormone-related protein receptor |
| UniProt Protein Name: | Parathyroid hormone/parathyroid hormone-related peptide receptor |
| UniProt Synonym Protein Names: | PTH/PTHrP type I receptor; PTH/PTHr receptor; Parathyroid hormone 1 receptor; PTH1 receptor |
| Protein Family: | Parathyroid hormone/parathyroid hormone-related peptide receptor |
| UniProt Gene Name: | PTH1R |
| UniProt Entry Name: | PTH1R_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PTH1R antibody (CAB1744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded Rat leg bone using PTH1R Rabbit pAb (CAB1744) at dilution of 1:100 (40x lens). |
Related Products
Anti-GALP Antibody (CAB17445)
