Anti-PSRC1 Antibody (CAB5484)

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SKU:
CAB5484
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-PSRC1 Antibody
Antibody SKU:CAB5484
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human PSRC1 (NP_001005290.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human PSRC1 (NP_001005290.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEDL EEDV RFIV DETL DFGG LSPS DSRE EEDI TVLV TPEK PLRR GLSH RSDP NAVA PAPQ GVRL SLGP LSPE KLEE ILDE ANRL AAQL EQCA LQDR ESAG EGLG PRRV KPSP RRET FVLK DSPV RDLL PTVN SLTR STPS PSSL TPRL RSND RKGS VRAL RATS GKRP SNMK RESP TCNL FPAS KSPA SSPL TRST PPVR GRAG PSGR AAAS EET
Gene ID:84722
Uniprot:Q6PGN9
Cellular Location:Cytoplasm, cytoskeleton, spindle, spindle pole
Calculated MW:16kDa/33kDa/35kDa/38kDa
Observed MW:39kDa
Synonyms:PSRC1, DDA3, FP3214
Background:This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:PSRC1: Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression. Belongs to the PSRC1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; spindle pole; spindle microtubule; cytoplasm; spindle; midbody; cytosol

Molecular Function:protein binding; microtubule binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of transcription, DNA-dependent; positive regulation of microtubule polymerization; negative regulation of cell growth; mitotic metaphase plate congression; microtubule bundle formation

NCBI Summary:This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Apr 2014]
UniProt Code:Q6PGN9
NCBI GenInfo Identifier:74737651
NCBI Gene ID:84722
NCBI Accession:Q6PGN9.1
UniProt Secondary Accession:Q6PGN9,Q5T2Z3, Q6ZTI8, Q71MG3, Q9BV77,
UniProt Related Accession:Q6PGN9
Molecular Weight:363
NCBI Full Name:Proline/serine-rich coiled-coil protein 1
NCBI Synonym Full Names:proline/serine-rich coiled-coil 1
NCBI Official Symbol:PSRC1  
NCBI Official Synonym Symbols:DDA3; FP3214  
NCBI Protein Information:proline/serine-rich coiled-coil protein 1; p53-regulated DDA3; differential display and activated by p53
UniProt Protein Name:Proline/serine-rich coiled-coil protein 1
Protein Family:Proline/serine-rich coiled-coil protein
UniProt Gene Name:PSRC1  
UniProt Entry Name:PSRC1_HUMAN
Anti-PSRC1 Antibody (CAB5484)Western blot analysis of extracts of various cell lines, using PSRC1 antibody (CAB5484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PSRC1 Antibody (CAB5484)Immunohistochemistry of paraffin-embedded human esophagus using PSRC1 antibody (CAB5484) at dilution of 1:100 (40x lens).
Anti-PSRC1 Antibody (CAB5484)Immunohistochemistry of paraffin-embedded mouse stomach using PSRC1 antibody (CAB5484) at dilution of 1:100 (40x lens).

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