Anti-PSME4 Antibody (CAB13815)
- SKU:
- CAB13815
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PSME4 Antibody |
| Antibody SKU: | CAB13815 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1634-1843 of human PSME4 (NP_055429.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, U-87MG, 293T, HeLa, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1634-1843 of human PSME4 (NP_055429.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LYPH QVPL VLQV LKQT ARSS SWHA RYTV LTYL QTMV FYNL FIFL NNED AVKD IRWL VISL LEDE QLEV REMA ATTL SGLL QCNF LTMD SPMQ IHFE QLCK TKLP KKRK RDPG SVGD TIPS AELV KRHA GVLG LGAC VLSS PYDV PTWM PQLL MNLS AHLN DPQP IEMT VKKT LSNF RRTH HDNW QEHK QQFT DDQL LVLT DLLV SPCY YA |
| Gene ID: | 23198 |
| Uniprot: | Q14997 |
| Cellular Location: | Cytoplasm, Nucleus, Nucleus speckle, cytosol |
| Calculated MW: | 24kDa/113kDa/139kDa/211kDa |
| Observed MW: | 211kDa |
| Synonyms: | PSME4, PA200 |
| Background: |
| UniProt Protein Function: | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. |
| UniProt Code: | Q14997 |
| NCBI GenInfo Identifier: | 163644283 |
| NCBI Gene ID: | 23198 |
| NCBI Accession: | NP_055429.2 |
| UniProt Secondary Accession: | Q14997,Q1XBG4, Q1XBG5, Q1XBG6, Q2M1Z0, Q6IPR2, Q86XF8 |
| UniProt Related Accession: | Q14997 |
| Molecular Weight: | Calculated: 24kDa; 113kDa; 139kDa; 211kDaObserved: 211kDa |
| NCBI Full Name: | proteasome activator complex subunit 4 |
| NCBI Synonym Full Names: | proteasome activator subunit 4 |
| NCBI Official Symbol: | PSME4 |
| NCBI Official Synonym Symbols: | PA200 |
| NCBI Protein Information: | proteasome activator complex subunit 4 |
| UniProt Protein Name: | Proteasome activator complex subunit 4 |
| UniProt Synonym Protein Names: | Proteasome activator PA200 |
| Protein Family: | Proteasome activator complex |
| UniProt Gene Name: | PSME4 |
 | Western blot analysis of extracts of various cell lines, using PSME4 antibody (CAB13815) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Mouse heart using PSME4 Rabbit pAb (CAB13815) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat kidney using PSME4 Rabbit pAb (CAB13815) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human esophageal using PSME4 Rabbit pAb (CAB13815) at dilution of 1:100 (40x lens). |
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