Anti-PSMC4 Antibody (CAB2505)
- SKU:
- CAB2505
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PSMC4 Antibody |
| Antibody SKU: | CAB2505 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human PSMC4 (NP_006494.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, U-251MG, 293T, BT-474, A-549, HeLa, Jurkat, Mouse liver, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human PSMC4 (NP_006494.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEEI GILV EKAQ DEIP ALSV SRPQ TGLS FLGP EPED LEDL YSRY KKLQ QELE FLEV QEEY IKDE QKNL KKEF LHAQ EEVK RIQS IPLV IGQF LEAV DQNT AIVG STTG SNYY VRIL STID RELL KPNA SVAL HKHS NALV DVLP PEAD SSIM MLTS DQKP DVMY A |
| Gene ID: | 5704 |
| Uniprot: | P43686 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 43kDa/47kDa |
| Observed MW: | 47kDa |
| Synonyms: | PSMC4, MIP224, RPT3, S6, TBP-7, TBP7, ATPase 4 |
| Background: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | RPT3: an ATPase subunit of the 26S proteasome. A member of the triple-A family of ATPases which have a chaperone-like activity. Interacts with an orphan member of the nuclear hormone receptor superfamily highly expressed in liver, and with gankyrin, a liver oncoprotein. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Protease; Proteasome complex; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 19q13.11-q13.13 Cellular Component: proteasome complex; nucleoplasm; membrane; cytoplasm; inclusion body; nucleus; cytosol Molecular Function:protein binding; ATPase activity; ATP binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; blastocyst development; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; proteolysis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
| UniProt Code: | P43686 |
| NCBI GenInfo Identifier: | 20532409 |
| NCBI Gene ID: | 5704 |
| NCBI Accession: | P43686.2 |
| UniProt Secondary Accession: | P43686,Q96FV5, Q9UBM3, Q9UEX3, |
| UniProt Related Accession: | P43686 |
| Molecular Weight: | 418 |
| NCBI Full Name: | 26S protease regulatory subunit 6B |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) 26S subunit, ATPase, 4 |
| NCBI Official Symbol: | PSMC4 |
| NCBI Official Synonym Symbols: | S6; RPT3; TBP7; TBP-7; MIP224 |
| NCBI Protein Information: | 26S protease regulatory subunit 6B; Tat-binding protein 7; protease 26S subunit 6; MB67-interacting protein; 26S proteasome AAA-ATPase subunit RPT3 |
| UniProt Protein Name: | 26S protease regulatory subunit 6B |
| UniProt Synonym Protein Names: | 26S proteasome AAA-ATPase subunit RPT3; MB67-interacting protein; MIP224; Proteasome 26S subunit ATPase 4; Tat-binding protein 7; TBP-7 |
| Protein Family: | 26S protease regulatory |
| UniProt Gene Name: | PSMC4 |
| UniProt Entry Name: | PRS6B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSMC4 antibody (CAB2505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using PSMC4 antibody (CAB2505). Blue: DAPI for nuclear staining. |
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