Anti-PSMA6 Antibody (CAB2188)
- SKU:
- CAB2188
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PSMA6 Antibody |
| Antibody SKU: | CAB2188 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PSMA6 (NP_002782.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa, A-549, COS-7, PC-12, MCF7, NCI-H460, A375, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PSMA6 (NP_002782.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSRG SSAG FDRH ITIF SPEG RLYQ VEYA FKAI NQGG LTSV AVRG KDCA VIVT QKKV PDKL LDSS TVTH LFKI TENI GCVM TGMT ADSR SQVQ RARY EAAN WKYK YGYE IPVD MLCK RIAD ISQV YTQN AEMR PLGC CMIL IGID EEQG PQVY KCDP AGYY CGFK ATAA GVKQ TEST SFLE KKVK KKFD WTFE QTVE TAIT CLST VLSI DFKP SEIE VGVV TVEN PKFR ILTE AEID AHLV ALAE RD |
| Gene ID: | 5687 |
| Uniprot: | P60900 |
| Cellular Location: | Cytoplasm, Nucleus, P-body |
| Calculated MW: | 18kDa/25kDa/27kDa |
| Observed MW: | 27kDa |
| Synonyms: | PSMA6, IOTA, PROS27, p27K |
| Background: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. |
| UniProt Protein Function: | PSMA6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Belongs to the peptidase T1A family. |
| UniProt Protein Details: | Protein type:Proteasome complex; EC 3.4.25.1; Protease Chromosomal Location of Human Ortholog: 14q13 Cellular Component: proteasome complex; nucleoplasm; polysome; proteasome core complex; sarcomere; nuclear matrix; myofibril; cytoplasm; nucleus; cytosol Molecular Function:threonine endopeptidase activity; NF-kappaB binding; protein binding; RNA binding; endopeptidase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of inflammatory response; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis Disease: Myocardial Infarction, Susceptibility To |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P60900 |
| NCBI GenInfo Identifier: | 46397659 |
| NCBI Gene ID: | 5687 |
| NCBI Accession: | P60900.1 |
| UniProt Secondary Accession: | P60900,P34062, Q6IB60, B2R7J9, B4DQR4, B4DXJ9, |
| UniProt Related Accession: | P60900 |
| Molecular Weight: | 246 |
| NCBI Full Name: | Proteasome subunit alpha type-6 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) subunit, alpha type, 6 |
| NCBI Official Symbol: | PSMA6 |
| NCBI Official Synonym Symbols: | IOTA; p27K; PROS27 |
| NCBI Protein Information: | proteasome subunit alpha type-6; PROS-27; macropain iota chain; prosomal P27K protein; proteasome iota chain; macropain subunit iota; 27 kDa prosomal protein; proteasome subunit iota; multicatalytic endopeptidase complex iota chain |
| UniProt Protein Name: | Proteasome subunit alpha type-6 |
| UniProt Synonym Protein Names: | 27 kDa prosomal protein; PROS-27; p27K; Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PSMA6 |
| UniProt Entry Name: | PSA6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSMA6 antibody (CAB2188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using PSMA6 antibody (CAB2188). Blue: DAPI for nuclear staining. |
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