Anti-PRPF4B Antibody (CAB14815)
- SKU:
- CAB14815
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-PRPF4B Antibody |
| Antibody SKU: | CAB14815 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human PRPF4B (NP_003904.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, HeLa, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 550-760 of human PRPF4B (NP_003904.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QKYK YLAE DSNM SVPS EPSS PQSS TRTR SPSP DDIL ERVA ADVK EYER ENVD TFEA SVKA KHNL MTVE QNNG SSQK KLLA PDMF TESD DMFA AYFD SARL RAAG IGKD FKEN PNLR DNWT DAEG YYRV NIGE VLDK RYNV YGYT GQGV FSNV VRAR DNAR ANQE VAVK IIRN NELM QKTG LKEL EFLK KLND ADPD DKFH CLRL FRHF YHK |
| Gene ID: | 8899 |
| Uniprot: | Q13523 |
| Cellular Location: | Nucleus |
| Calculated MW: | 116kDa |
| Observed MW: | 160kDa |
| Synonyms: | PRPF4B, PR4H, PRP4, PRP4H, PRP4K, dJ1013A10.1 |
| Background: | Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). |
| UniProt Protein Function: | PRP4: a kinase of the DYRK family associated with the spliceosome and the kinetochore. It binds pre-mRNA splicing factors SFRS8, PRP6 and pinin. It is an essential kinase that associates with both the U5 snRNP and the N-CoR deacetylase complexes. It may help coordinate pre-mRNA splicing with chromatin remodeling and transcriptional regulation. PRP4 has recently been shown to be a kinetochore component that is necessary to establish a functional spindle assembly checkpoint, and is necessary for recruitment or maintenance of the checkpoint proteins RPS27, MAD1, and MAD2 at the kinetochores. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; RNA splicing; Spliceosome; EC 2.7.11.1; Protein kinase, CMGC; CMGC group; DYRK family; PRP4 subfamily Chromosomal Location of Human Ortholog: 6p25.2 Cellular Component: nucleoplasm Molecular Function:protein binding; protein kinase activity Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing |
| NCBI Summary: | Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13523 |
| NCBI GenInfo Identifier: | 317373526 |
| NCBI Gene ID: | 8899 |
| NCBI Accession: | Q13523.3 |
| UniProt Secondary Accession: | Q13523,Q5D0F6, Q5TAY8, Q8IVC3, Q8TDP2, Q96QT7, Q9UEE6 A8K5C9, |
| UniProt Related Accession: | Q13523 |
| Molecular Weight: | 116,987 Da |
| NCBI Full Name: | Serine/threonine-protein kinase PRP4 homolog |
| NCBI Synonym Full Names: | pre-mRNA processing factor 4B |
| NCBI Official Symbol: | PRPF4B |
| NCBI Official Synonym Symbols: | PR4H; PRP4; PRP4H; PRP4K; dJ1013A10.1 |
| NCBI Protein Information: | serine/threonine-protein kinase PRP4 homolog |
| UniProt Protein Name: | Serine/threonine-protein kinase PRP4 homolog |
| UniProt Synonym Protein Names: | PRP4 kinase; PRP4 pre-mRNA-processing factor 4 homolog |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | PRPF4B |
| UniProt Entry Name: | PRP4B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRPF4B antibody (CAB14815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-FAM160B2 Antibody (CAB18835)
