Anti-Protein O-GlcNAcase Antibody (CAB9033)
- SKU:
- CAB9033
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| Antibody Name: | Anti-Protein O-GlcNAcase Antibody |
| Antibody SKU: | CAB9033 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A375, BxPC-3, HeLa, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YQEP IMSQ GAAL SGEP TTLT KEEE KKQP DEEP MDMV VEKQ EETD HKND NQIL SEIV EAKM AEEL KPMD TDKE SIAE SKSP EMSM QEDC ISDI APMQ TDEQ TNKE QFVP GPNE KPLY TAEP VTLE DLQL LADL FYLP YEHG PKGA QMLR EFQW LRAN SSVV SVNC KGKD SEKI EEWR SRAA KFEE MCGL VMGM FTRL SNCA N |
| Gene ID: | 10724 |
| Uniprot: | O60502 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 76kDa/95kDa/96kDa/102kDa |
| Observed MW: | 120kDa |
| Synonyms: | MGEA5, MEA5, NCOAT, OGA |
| Background: |
| UniProt Protein Function: | OGA: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Acetyltransferase; Hydrolase; EC 3.2.1.169; EC 3.2.1.52 Chromosomal Location of Human Ortholog: 10q24.1-q24.3 Cellular Component: membrane; cytosol; nucleus Molecular Function:beta-N-acetylglucosaminidase activity; histone acetyltransferase activity; hyalurononglucosaminidase activity Biological Process: positive regulation of DNA metabolic process; positive regulation of protein complex disassembly; dATP metabolic process; positive regulation of proteolysis; protein deglycosylation; N-acetylglucosamine metabolic process; positive regulation of insulin secretion; positive regulation of growth hormone secretion; positive regulation of glucose import; negative regulation of protein amino acid glycosylation; positive regulation of mitochondrial depolarization; response to steroid hormone stimulus; positive regulation of cell killing; glycoprotein catabolic process; histone acetylation; protein targeting to membrane; aging |
| NCBI Summary: | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O60502 |
| NCBI GenInfo Identifier: | 215490056 |
| NCBI Gene ID: | 10724 |
| NCBI Accession: | NP_001135906.1 |
| UniProt Secondary Accession: | O60502,O75166, Q86WV0, Q8IV98, Q9BVA5, Q9HAR0, B7WPB9 D3DR79, E9PGF9, |
| UniProt Related Accession: | O60502 |
| Molecular Weight: | 96,932 Da |
| NCBI Full Name: | bifunctional protein NCOAT isoform b |
| NCBI Synonym Full Names: | meningioma expressed antigen 5 (hyaluronidase) |
| NCBI Official Symbol: | MGEA5 |
| NCBI Official Synonym Symbols: | OGA; MEA5; NCOAT |
| NCBI Protein Information: | bifunctional protein NCOAT; O-GlcNAcase; hyaluronidase in meningioma; meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase |
| UniProt Protein Name: | Bifunctional protein NCOAT |
| UniProt Synonym Protein Names: | Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferaseIncluding the following 2 domains:Protein O-GlcNAcase (EC:3.2.1.169)Alternative name(s):Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA |
| Protein Family: | Protein O-GlcNAcase |
| UniProt Gene Name: | MGEA5 |
| UniProt Entry Name: | NCOAT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MGEA5 antibody (CAB9033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Human MGEA5 (Protein O-GlcNAcase) ELISA Kit (HUFI08724)
