Anti-Protein MEMO1 Antibody (CAB8582)
- SKU:
- CAB8582
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Protein MEMO1 Antibody |
| Antibody SKU: | CAB8582 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human MEMO1 (NP_057039.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Mouse spleen, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human MEMO1 (NP_057039.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSNR VVCR EASH AGSW YTAS GPQL NAQL EGWL SQVQ STKR PARA IIAP HAGY TYCG SCAA HAYK QVDP SITR RIFI LGPS HHVP LSRC ALSS VDIY RTPL YDLR IDQK IYGE LWKT GMFE RMSL QTDE DEHS IEMH LPYT AKAM ESHK DEFT IIPV LVGA LSES KEQE FGKL FSKY LADP SNLF VVSS DFCH WGQR FRYS YYDE SQGE IYRS IEHL DKMG MSII EQLD PVSF SNYL KKYH NTIC GRHP IGVL LNAI TELQ KNGM NMSF SFLN YAQS SQCR NWQD SSVS YAAG ALTV H |
| Gene ID: | 51072 |
| Uniprot: | Q9Y316 |
| Cellular Location: | |
| Calculated MW: | 31kDa/33kDa/34kDa |
| Observed MW: | 32kDa |
| Synonyms: | MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7 |
| Background: |
| UniProt Protein Function: | MEMO1: a highly conserved protein that controls cell migration by relaying extracellular chemotactic signals to the microtubule cytoskeleton. Regulates directionality during HER2-dependent cell migration. Controls microtubules, the actin network, and adhesion site formation in migrating cells via the MEMO1-RHOA-DIAPH1 signaling pathway. Homologous to class III nonheme iron-dependent dioxygenases. No metal-binding or enzymatic activity has been detected. Binds HER2 phosphorylated at Y1222 via its vestigial enzymatic active site. Binds to and influences insulin-like growth factor-I (IRS1) receptor-dependent signaling events in breast carcinogenesis. Influences the epithelial-mesenchymal transition (EMT) via the IRS1/PI3K/Akt/Snail1 pathway. Interacts with cofilin, and influences PLCg1 and cofilin activities. Increased expression is associated with cancer aggressiveness. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Two isoform of the human protein are produced by alternatuve splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p22-p21 Cellular Component: cytosol; nucleus Molecular Function:protein binding Biological Process: regulation of microtubule-based process |
| UniProt Code: | Q9Y316 |
| NCBI GenInfo Identifier: | 17511761 |
| NCBI Gene ID: | 51072 |
| NCBI Accession: | BC018733 |
| UniProt Secondary Accession: | Q9Y316,Q5R2V8, Q5R2V9, Q6NSL5, B4DLS0, D6W575, |
| UniProt Related Accession: | Q9Y316 |
| Molecular Weight: | 34,294 Da |
| NCBI Full Name: | Homo sapiens mediator of cell motility 1, mRNA |
| NCBI Synonym Full Names: | mediator of cell motility 1 |
| NCBI Official Symbol: | MEMO1 |
| NCBI Official Synonym Symbols: | MEMO; C2orf4; CGI-27; NS5ATP7 |
| NCBI Protein Information: | protein MEMO1 |
| UniProt Protein Name: | Protein MEMO1 |
| UniProt Synonym Protein Names: | C21orf19-like protein; Hepatitis C virus NS5A-transactivated protein 7; HCV NS5A-transactivated protein 7; Mediator of ErbB2-driven cell motility 1; Mediator of cell motility 1; Memo-1 |
| Protein Family: | MEMO1 family protein |
| UniProt Gene Name: | MEMO1 |
| UniProt Entry Name: | MEMO1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MEMO1 antibody (CAB8582) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human prostate using MEMO1 antibody (CAB8582) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using MEMO1 antibody (CAB8582) at dilution of 1:100 (40x lens). |
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