Anti-Protein Jumonji Antibody (CAB9823)
- SKU:
- CAB9823
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Protein Jumonji Antibody |
| Antibody SKU: | CAB9823 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1027-1246 of human JARID2 (NP_004964.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, Mouse thymus, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1027-1246 of human JARID2 (NP_004964.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ETVH FATT QWTS MGFE TAKE MKRR HIAK PFSM EKLL YQIA QAEA KKEN GPTL STIS ALLD ELRD TELR QRRQ LFEA GLHS SARY GSHD GSST VADG KKKP RKWL QLET SERR CQIC QHLC YLSM VVQE NENV VFCL ECAL RHVE KQKS CRGL KLMY RYDE EQII SLVN QICG KVSG KNGS IENC LSKP TPKR GPRK RATV DVPP SRLS ASSS SKSA SSSS |
| Gene ID: | 3720 |
| Uniprot: | Q92833 |
| Cellular Location: | Nucleus |
| Calculated MW: | 106kDa/119kDa/138kDa |
| Observed MW: | 160kDa |
| Synonyms: | JARID2, JMJ |
| Background: | This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. |
| UniProt Protein Function: | JARID2: a Polycomb protein required for neural tube formation. Contains a Jumonji C domain but lacks histone demethylase activity. Essential for normal heart development and function. Required for embryonic viability. Down-regulation of Jarid2 impairs ES cell differentiation and Oct4 silencing. Associates with the Polycomb-Repressive Complex 2 (PRC2), stimulating PRC2 methyltransferase activity. PRC2 includes the histone H3 Lys 27 (H3K27) methyltransferase Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells. The ability of Jarid 2 to be recruited to DNA in ES cells is interdependent on PRC2. Its knockdown reduces PRC2 at its target promoters, while ES cells lacking the PRC2 component EED are deficient in Jarid2 promoter activity. Acts as a transcriptional repressor of ANF by binding to both GATA4 and NKX2-5 and repressing their transcriptional activator activities. Participates in the negative regulation of cell proliferation signaling. Interacts with the N-terminal region of GATA4 and the C- terminal region of the NKX2-5 homeobox and represses their ability to activate transcription of ANF. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 6p24-p23 Cellular Component: ESC/E(Z) complex; histone methyltransferase complex; nucleoplasm; nucleus Molecular Function:chromatin binding; histone demethylase activity Biological Process: central nervous system development; chromatin modification; liver development; negative regulation of cell proliferation; negative regulation of gene expression, epigenetic; negative regulation of histone methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K9 methylation; spleen development; stem cell differentiation; thymus development; transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012] |
| UniProt Code: | Q92833 |
| NCBI GenInfo Identifier: | 11863152 |
| NCBI Gene ID: | 3720 |
| NCBI Accession: | NP_004964.2 |
| UniProt Secondary Accession: | Q92833,Q5U5L5, Q86X63, A8K9Z6, B7Z5S5, B7Z8L0, |
| UniProt Related Accession: | Q92833 |
| Molecular Weight: | 119,689 Da |
| NCBI Full Name: | protein Jumonji isoform 1 |
| NCBI Synonym Full Names: | jumonji and AT-rich interaction domain containing 2 |
| NCBI Official Symbol: | JARID2 |
| NCBI Official Synonym Symbols: | JMJ |
| NCBI Protein Information: | protein Jumonji |
| UniProt Protein Name: | Protein Jumonji |
| UniProt Synonym Protein Names: | Jumonji/ARID domain-containing protein 2 |
| UniProt Gene Name: | JARID2 |
| UniProt Entry Name: | JARD2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using JARID2 antibody (CAB9823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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