Anti-PRMT8 Antibody (CAB18440)[KO Validated]

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SKU:
CAB18440
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-PRMT8 Antibody [KO Validated]
Antibody SKU:CAB18440
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PRMT8.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse brain, Rat brain
Immunogen:Recombinant protein of human PRMT8.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:56341
Uniprot:Q9NR22
Cellular Location:Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:44kDa/45kDa
Observed MW:42KDa
Anti-PRMT8 Antibody [KO Validated] (CAB18440)Western blot analysis of extracts of various cell lines, using PRMT8 antibody (CAB18440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-PRMT8 Antibody [KO Validated] (CAB18440)Western blot analysis of extracts from normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells, using PRMT8 Rabbit pAb antibody (CAB18440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:HRMT1L3, HRMT1L4, PRMT8
Background:Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
UniProt Protein Function:PRMT8: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS. Belongs to the protein arginine N-methyltransferase family. PRMT8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase, protein arginine; EC 2.1.1.-; Methyltransferase

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cytosol; nucleus; plasma membrane

Molecular Function:histone-arginine N-methyltransferase activity; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; regulation of protein binding; regulation of transcription, DNA-dependent

NCBI Summary:Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9NR22
NCBI GenInfo Identifier:88983969
NCBI Gene ID:56341
NCBI Accession:Q9NR22.2
UniProt Secondary Accession:Q9NR22,Q8TBJ8, B2RDP0,
UniProt Related Accession:Q9NR22
Molecular Weight:44,169 Da
NCBI Full Name:Protein arginine N-methyltransferase 8
NCBI Synonym Full Names:protein arginine methyltransferase 8
NCBI Official Symbol:PRMT8  
NCBI Official Synonym Symbols:HRMT1L3; HRMT1L4  
NCBI Protein Information:protein arginine N-methyltransferase 8
UniProt Protein Name:Protein arginine N-methyltransferase 8
UniProt Synonym Protein Names:Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
Protein Family:Protein arginine N-methyltransferase
UniProt Gene Name:PRMT8  
UniProt Entry Name:ANM8_HUMAN

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