Anti-PRKX 항체 (CAB15305)

Antibody Name:	Anti-PRKX Antibody
Antibody SKU:	CAB15305
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 239-358 of human PRKX (NP_005035.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human
Positive Samples:	A549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 239-358 of human PRKX (NP_005035.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGFP PFFD DNPF GIYQ KILA GKID FPRH LDFH VKDL IKKL LVVD RTRR LGNM KNGA NDVK HHRW FRSV DWEA VPQR KLKP PIVP KIAG DGDT SNFE TYPE NDWD TAAP VPQK DLEI FKNF
Gene ID:	5613
Uniprot:	P51817
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	40kDa
Observed MW:	41kDa

Synonyms:

PRKX, PKX1

Background:

This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y.

UniProt Protein Function:	PRKX: Serine/threonine protein kinase regulated by and mediating cAMP signaling in cells. Acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation. A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
UniProt Protein Details:	Protein type:Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; PKA family Chromosomal Location of Human Ortholog: Xp22.3 Cellular Component: cytoplasm; nucleus Molecular Function:cAMP-dependent protein kinase activity; protein binding Biological Process: angiogenesis; cell adhesion; cell-substrate adhesion; endothelial cell migration; endothelial cell proliferation; myeloid cell differentiation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; regulation of cell adhesion; regulation of cell migration
NCBI Summary:	This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010]
UniProt Code:	P51817
NCBI GenInfo Identifier:	1709648
NCBI Gene ID:	5613
NCBI Accession:	P51817.1
UniProt Related Accession:	P51817
Molecular Weight:	40,896 Da
NCBI Full Name:	cAMP-dependent protein kinase catalytic subunit PRKX
NCBI Synonym Full Names:	protein kinase, X-linked
NCBI Official Symbol:	PRKX
NCBI Official Synonym Symbols:	PKX1
NCBI Protein Information:	cAMP-dependent protein kinase catalytic subunit PRKX
UniProt Protein Name:	cAMP-dependent protein kinase catalytic subunit PRKX
UniProt Synonym Protein Names:	Protein kinase PKX1
Protein Family:	cAMP-dependent protein kinase
UniProt Gene Name:	PRKX
UniProt Entry Name:	PRKX_HUMAN

Western blot analysis of extracts of A549 cells, using PRKX antibody (CAB15305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

PRKX Antibody (PACO04773)

PRKX Antibody (PACO11482)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)