Anti-PRDX5 항체 (CAB6329)

Antibody Name:	Anti-PRDX5 Antibody
Antibody SKU:	CAB6329
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 53-214 of human PRDX5 (NP_036226.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 53-214 of human PRDX5 (NP_036226.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPI KVGD AIPA VEVF EGEP GNKV NLAE LFKG KKGV LFGV PGAF TPGC SKTH LPGF VEQA EALK AKGV QVVA CLSV NDAF VTGE WGRA HKAE GKVR LLAD PTGA FGKE TDLL LDDS LVSI FGNR RLKR FSMV VQDG IVKA LNVE PDGT GLTC SLAP NIIS QL
Gene ID:	25824
Uniprot:	P30044
Cellular Location:	Cytoplasm, Mitochondrion, Peroxisome
Calculated MW:	12kDa/17kDa/22kDa
Observed MW:	17KDa

Synonyms:

PRDX5, ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V

Background:

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	PRDX5: Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. Belongs to the peroxiredoxin 2 family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 1.11.1.15; Oxidoreductase Chromosomal Location of Human Ortholog: 11q13 Cellular Component: peroxisomal matrix; extracellular space; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; mitochondrial matrix; cytoplasm; peroxisome; cytoplasmic vesicle; cytosol; nucleus Molecular Function:antioxidant activity; protein dimerization activity; peroxidase activity; peroxiredoxin activity; caspase inhibitor activity; receptor binding Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; negative regulation of transcription from RNA polymerase III promoter; positive regulation of collagen biosynthetic process; negative regulation of oxidoreductase activity; response to oxidative stress; negative regulation of caspase activity; inflammatory response; negative regulation of apoptosis
NCBI Summary:	This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P30044
NCBI GenInfo Identifier:	317373539
NCBI Gene ID:	25824
NCBI Accession:	P30044.4
UniProt Secondary Accession:	P30044,Q14CK0, Q6IAF2, Q9UBU5, Q9UJU4, Q9UKX4, A6NC19 A6NG06, B7ZLJ4, B7ZVW3,
UniProt Related Accession:	P30044
Molecular Weight:	12,897 Da
NCBI Full Name:	Peroxiredoxin-5, mitochondrial
NCBI Synonym Full Names:	peroxiredoxin 5
NCBI Official Symbol:	PRDX5
NCBI Official Synonym Symbols:	PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
NCBI Protein Information:	peroxiredoxin-5, mitochondrial; TPx type VI; peroxiredoxin V; Alu co-repressor 1; thioredoxin reductase; antioxidant enzyme B166; thioredoxin peroxidase PMP20; liver tissue 2D-page spot 71B; peroxisomal antioxidant enzyme; epididymis secretory protein Li 55
UniProt Protein Name:	Peroxiredoxin-5, mitochondrial
UniProt Synonym Protein Names:	Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase
Protein Family:	Peroxiredoxin
UniProt Gene Name:	PRDX5
UniProt Entry Name:	PRDX5_HUMAN

	Western blot analysis of extracts of various cell lines, using PRDX5 antibody (CAB6329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts of Mouse lung, using PRDX5 antibody (CAB6329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded mouse kidney using PRDX5 antibody (CAB6329) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using PRDX5 Rabbit pAb (CAB6329) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PRDX5 Antibody (CAB1269)	Human PRDX5 / Peroxiredoxin-5 ELISA Kit
Anti-Peroxiredoxin 5 Antibody (CAB3954)	Human Peroxiredoxin-5, mitochondrial (PRDX5) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)