Anti-PRDM6 Antibody (CAB7846)

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SKU:
CAB7846
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-PRDM6 Antibody
Antibody SKU:CAB7846
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 376-595 of human PRDM6 (NP_001129711.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, MCF7, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 376-595 of human PRDM6 (NP_001129711.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQCI AQDE NLNV PSTV MEAM CRQD ALQP FNKS SKLA PTTQ QRSV VFPQ TPCS RNFS LLDK SGPI ESGF NQIN VKNQ RVLA SPTS TSQL HSEF SDWH LWKC GQCF KTFT QRIL LQMH VCTQ NPDR PYQC GHCS QSFS QPSE LRNH VVTH SSDR PFKC GYCG RAFA GATT LNNH IRTH TGEK PFKC ERCE RSFT QATQ LSRH QRMP NECK PITE SPES IEVD
Gene ID:93166
Uniprot:Q9NQX0
Cellular Location:Nucleus
Calculated MW:14kDa/46kDa/64kDa
Observed MW:80kDa
Synonyms:PRDM6, KMT8C, PDA3, PRISM
Background:
UniProt Protein Function:PRDM6: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell development/differentiation; Methyltransferase; C2H2-type zinc finger protein; Methyltransferase, protein lysine; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 5q23.2

UniProt Code:Q9NQX0
NCBI GenInfo Identifier:223590133
NCBI Gene ID:93166
NCBI Accession:Q9NQX0.2
UniProt Secondary Accession:Q9NQX0,Q9NQW9, B5MCJ4,
UniProt Related Accession:Q9NQX0
Molecular Weight:46,635 Da
NCBI Full Name:Putative histone-lysine N-methyltransferase PRDM6
NCBI Synonym Full Names:PR/SET domain 6
NCBI Official Symbol:PRDM6  
NCBI Official Synonym Symbols:PRISM  
NCBI Protein Information:putative histone-lysine N-methyltransferase PRDM6
UniProt Protein Name:Putative histone-lysine N-methyltransferase PRDM6
UniProt Synonym Protein Names:PR domain zinc finger protein 6; PR domain-containing protein 6
Protein Family:Putative histone-lysine N-methyltransferase
UniProt Gene Name:PRDM6  
UniProt Entry Name:PRDM6_HUMAN
Anti-PRDM6 Antibody (CAB7846)Western blot analysis of extracts of various cell lines, using PRDM6 antibody (CAB7846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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