Anti-PRAME Antibody (CAB14507)
- SKU:
- CAB14507
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PRAME Antibody |
| Antibody SKU: | CAB14507 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U2OS, U-251MG, Mouse liver, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRAME (NP_006106.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MERR RLWG SIQS RYIS MSVW TSPR RLVE LAGQ SLLK DEAL AIAA LELL PREL FPPL FMAA FDGR HSQT LKAM VQAW PFTC LPLG VLMK GQHL HLET FKAV LDGL DVLL AQEV RPRR WKLQ VLDL RKNS HQDF WTVW SGNR ASLY SFPE PEAA QPMT KKRK VDGL STEA EQPF IPVE VLVD LFLK EGAC DELF SYLI EKVK |
| Gene ID: | 23532 |
| Uniprot: | P78395 |
| Cellular Location: | Cell membrane, Nucleus |
| Calculated MW: | 57kDa |
| Observed MW: | 58kDa |
| Synonyms: | PRAME, CT130, MAPE, OIP-4, OIP4 |
| Background: | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Cancer Testis Antigen (CTA); Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 22q11.22 Molecular Function:protein binding; retinoic acid receptor binding Biological Process: negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation |
| NCBI Summary: | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| UniProt Code: | P78395 |
| NCBI GenInfo Identifier: | 6685631 |
| NCBI Gene ID: | 23532 |
| NCBI Accession: | P78395.1 |
| UniProt Secondary Accession: | P78395,O43481, Q8IXN8, B2R6Y7, |
| UniProt Related Accession: | P78395 |
| Molecular Weight: | 57,890 Da |
| NCBI Full Name: | Melanoma antigen preferentially expressed in tumors |
| NCBI Synonym Full Names: | preferentially expressed antigen in melanoma |
| NCBI Official Symbol: | PRAME |
| NCBI Official Synonym Symbols: | MAPE; OIP4; CT130; OIP-4 |
| NCBI Protein Information: | melanoma antigen preferentially expressed in tumors |
| UniProt Protein Name: | Melanoma antigen preferentially expressed in tumors |
| UniProt Synonym Protein Names: | Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma |
| Protein Family: | Melanoma antigen |
| UniProt Gene Name: | PRAME |
| UniProt Entry Name: | PRAME_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRAME antibody (CAB14507) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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