Anti-PPP3R1 Antibody (CAB0954)
- SKU:
- CAB0954
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PPP3R1 Antibody |
| Antibody SKU: | CAB0954 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_000936.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse spleen, Mouse lung, Rat brain, SH-SY5Y, SW480, HL-60, MCF7, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 11-170 of human PPP3R1 (NP_000936.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MCSH FDAD EIKR LGKR FKKL DLDN SGSL SVEE FMSL PELQ QNPL VQRV IDIF DTDG NGEV DFKE FIEG VSQF SVKG DKEQ KLRF AFRI YDMD KDGY ISNG ELFQ VLKM MVGN NLKD TQLQ QIVD KTII NADK DGDG RISF EEFC AVVG GLDI HKKM VVDV |
| Gene ID: | 5534 |
| Uniprot: | P63098 |
| Cellular Location: | Cell membrane, Cytoplasm, Lipid-anchor, Membrane, cytosol, sarcolemma |
| Calculated MW: | 19kDa |
| Observed MW: | 15kDa |
| Synonyms: | PPP3R1, CALNB1, CNB, CNB1 |
| Background: |
| UniProt Protein Function: | PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family. |
| UniProt Protein Details: | Protein type:Protein phosphatase, regulatory subunit; Cell development/differentiation; Apoptosis; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p15 Cellular Component: nucleoplasm; cytosol; calcineurin complex; sarcolemma Molecular Function:calmodulin binding; protein domain specific binding; protein binding; calcium-dependent protein serine/threonine phosphatase activity; calcium ion binding Biological Process: calcineurin-NFAT signaling pathway; positive regulation of NFAT protein import into nucleus; dephosphorylation; apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | P63098 |
| NCBI GenInfo Identifier: | 52000904 |
| NCBI Gene ID: | 5534 |
| NCBI Accession: | P63098.2 |
| UniProt Related Accession: | P63098 |
| Molecular Weight: | |
| NCBI Full Name: | Calcineurin subunit B type 1 |
| NCBI Synonym Full Names: | protein phosphatase 3 regulatory subunit B, alpha |
| NCBI Official Symbol: | PPP3R1 |
| NCBI Official Synonym Symbols: | CNB; CNB1; CALNB1 |
| NCBI Protein Information: | calcineurin subunit B type 1 |
| UniProt Protein Name: | Calcineurin subunit B type 1 |
| UniProt Synonym Protein Names: | Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1 |
| UniProt Gene Name: | PPP3R1 |
| UniProt Entry Name: | CANB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP3R1 antibody (CAB0954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human prostate using PPP3R1 antibody (CAB0954) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH/3T3 cells using PPP3R1 antibody (CAB0954) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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