Anti-PPP2R5D Antibody (CAB15707)

(No reviews yet) Write a Review
SKU:
CAB15707
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-PPP2R5D Antibody
Antibody SKU:CAB15707
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PPP2R5D (NP_006236.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, 293T, HeLa, Mouse heart, Mouse kidney, Rat kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PPP2R5D (NP_006236.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LYRN SKSH WNKT IHGL IYNA LKLF MEMN QKLF DDCT QQYK AEKQ KGRF RMKE REEM WQKI EELA RLNP QYPM FRAP PPLP PVYS METE TPTA EDIQ LLKR T
Gene ID:5528
Uniprot:Q14738
Cellular Location:Cytoplasm, Nucleus
Calculated MW:58kDa/66kDa/69kDa
Observed MW:80kDa
Synonyms:PPP2R5D, B56D, MRD35, B56delta
Background:The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:PPP2R5D: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. By retinoic acid; in neuroblastoma cell lines. Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. Belongs to the phosphatase 2A regulatory subunit B56 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:protein binding; protein serine/threonine phosphatase activity

Biological Process: nervous system development

Disease: Mental Retardation, Autosomal Dominant 35

NCBI Summary:The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q14738
NCBI GenInfo Identifier:7387495
NCBI Gene ID:5528
NCBI Accession:Q14738.1
UniProt Secondary Accession:Q14738,O00494, O00696, Q15171, Q5TC39, A8K3I9, B5BUA6
UniProt Related Accession:Q14738
Molecular Weight:58,453 Da
NCBI Full Name:Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
NCBI Synonym Full Names:protein phosphatase 2 regulatory subunit B'delta
NCBI Official Symbol:PPP2R5D  
NCBI Official Synonym Symbols:B56D; MRD35  
NCBI Protein Information:serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
UniProt Synonym Protein Names:PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta
Protein Family:Serine/threonine-protein phosphatase
UniProt Gene Name:PPP2R5D  
UniProt Entry Name:2A5D_HUMAN
Anti-PPP2R5D Antibody (CAB15707)Western blot analysis of extracts of various cell lines, using PPP2R5D antibody (CAB15707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PPP2R5D Antibody (CAB15707)Immunohistochemistry of paraffin-embedded rat brain using PPP2R5D antibody (CAB15707) at dilution of 1:100 (40x lens).
Anti-PPP2R5D Antibody (CAB15707)Immunohistochemistry of paraffin-embedded human thyroid cancer using PPP2R5D antibody (CAB15707) at dilution of 1:100 (40x lens).
Anti-PPP2R5D Antibody (CAB15707)Immunohistochemistry of paraffin-embedded mouse kidney using PPP2R5D antibody (CAB15707) at dilution of 1:100 (40x lens).
Anti-PPP2R5D Antibody (CAB15707)Immunofluorescence analysis of L929 cells using PPP2R5D antibody (CAB15707) at dilution of 1:100. Blue: DAPI for nuclear staining.

0 Reviews

View AllClose

Related Products