Anti-PPP2R4 Antibody (CAB11999)
- SKU:
- CAB11999
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PPP2R4 Antibody |
| Antibody SKU: | CAB11999 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 52-230 of human PPP2R4 (NP_821067.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, K-562, U-87MG, MCF7, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 52-230 of human PPP2R4 (NP_821067.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ILTL NEGV KGKK LTFE YRVS EAIE KLVA LLNT LDRW IDET PPVD QPSR FGNK AYRT WYAK LDEE AENL VATV VPTH LAAA VPEV AVYL KESV GNST RIDY GTGH EAAF AAFL CCLC KIGV LRVD DQIA IVFK VFNR YLEV MRKL QKTY RMEP AGSQ GVWG LDDF QFLP FIWG SSQL IDH |
| Gene ID: | 5524 |
| Uniprot: | Q15257 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 31kDa/33kDa/36kDa/40kDa |
| Observed MW: | 40kDa |
| Synonyms: | PTPA, PP2A, PPP2R4, PR53 |
| Background: | Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | PPP2R4: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A. Associates with PP2A heterodimeric core enzyme PP2A(D), composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). Interacts with PPP2CB. Widely expressed. Belongs to the PTPA-type PPIase family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; EC 5.2.1.8; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 9q34 Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytoplasm; nucleus Molecular Function:peptidyl-prolyl cis-trans isomerase activity; protein homodimerization activity; protein phosphatase 2A binding; protein heterodimerization activity; ATPase activity; protein phosphatase type 2A regulator activity; ATP binding; protein tyrosine phosphatase activator activity; receptor binding Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of protein amino acid dephosphorylation; protein peptidyl-prolyl isomerization; positive regulation of apoptosis; metabolic process; mitotic spindle organization and biogenesis in nucleus; negative regulation of phosphoprotein phosphatase activity; regulation of phosphoprotein phosphatase activity; positive regulation of protein amino acid dephosphorylation |
| NCBI Summary: | Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15257 |
| NCBI GenInfo Identifier: | 116242737 |
| NCBI Gene ID: | 5524 |
| NCBI Accession: | Q15257.3 |
| UniProt Related Accession: | Q15257 |
| Molecular Weight: | |
| NCBI Full Name: | Serine/threonine-protein phosphatase 2A activator |
| NCBI Synonym Full Names: | protein phosphatase 2 phosphatase activator |
| NCBI Official Symbol: | PTPA |
| NCBI Official Synonym Symbols: | PP2A; PR53; PPP2R4 |
| NCBI Protein Information: | serine/threonine-protein phosphatase 2A activator |
| UniProt Protein Name: | Serine/threonine-protein phosphatase 2A activator |
| UniProt Synonym Protein Names: | PP2A, subunit B', PR53 isoform; Phosphotyrosyl phosphatase activator; PTPA; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B' |
| Protein Family: | Prolyl tripeptidyl peptidase |
| UniProt Gene Name: | PPP2R4 |
| UniProt Entry Name: | PTPA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP2R4 antibody (CAB11999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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