Anti-PPP1R9B Antibody (CAB17230)
- SKU:
- CAB17230
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PPP1R9B Antibody |
| Antibody SKU: | CAB17230 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human PPP1R9B. |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, K-562, Mouse brain |
| Immunogen: | A synthetic peptide of human PPP1R9B. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 84687 |
| Uniprot: | Q96SB3 |
| Cellular Location: | |
| Calculated MW: | 89kDa |
| Observed MW: | 127kDa |
| Synonyms: | PPP1R9B, PPP1R6, PPP1R9, SPINO, Spn |
| Background: | This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. |
| UniProt Protein Function: | Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain. |
| UniProt Protein Details: | Protein type:Protein phosphatase, regulatory subunit; Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q21.33 Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium Molecular Function:protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding Biological Process: regulation of synaptic transmission; regulation of protein amino acid phosphorylation; cell migration; regulation of cell growth by extracellular stimulus; filopodium formation; RNA splicing; regulation of exit from mitosis; calcium-mediated signaling; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest |
| NCBI Summary: | This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014] |
| UniProt Code: | Q96SB3 |
| NCBI GenInfo Identifier: | 90101416 |
| NCBI Gene ID: | 84687 |
| NCBI Accession: | Q96SB3.2 |
| UniProt Secondary Accession: | Q96SB3,Q8TCR9, |
| UniProt Related Accession: | Q96SB3 |
| Molecular Weight: | 815 |
| NCBI Full Name: | Neurabin-2 |
| NCBI Synonym Full Names: | protein phosphatase 1, regulatory subunit 9B |
| NCBI Official Symbol: | PPP1R9B |
| NCBI Official Synonym Symbols: | Spn; SPINO; PPP1R6; PPP1R9 |
| NCBI Protein Information: | neurabin-2; neurabin-II; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin |
| UniProt Protein Name: | Neurabin-2 |
| UniProt Synonym Protein Names: | Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin |
| UniProt Gene Name: | PPP1R9B |
| UniProt Entry Name: | NEB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP1R9B antibody (CAB17230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
 | Immunofluorescence analysis of L929 cells using PPP1R9B antibody (CAB17230) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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