Anti-PPP1R1B Antibody (CAB2580)
- SKU:
- CAB2580
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PPP1R1B Antibody |
| Antibody SKU: | CAB2580 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human PPP1R1B (NP_001229393.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human PPP1R1B (NP_001229393.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLFR LSEH SSPE EEAS PHQR ASGE GHHL KSKR PNPC AYTP PSLK AVQR IAES HLQS ISNL NENQ ASEE EDEL GELR ELGY PREE DEEE EEDD EEEE EEED SQAE VLKV IRQS AGQK TTCG QGLE GPWE RPPP LDES ERDG GSED QVED PALS EPGE EPQR PSPS EPGT |
| Gene ID: | 84152 |
| Uniprot: | Q9UD71 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 18kDa/22kDa |
| Observed MW: | 32kDa |
| Synonyms: | PPP1R1B, DARPP-32, DARPP32 |
| Background: | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cell soma; cytoplasm; cytosol; nucleus Molecular Function:D1 dopamine receptor binding; D2 dopamine receptor binding; D3 dopamine receptor binding; D4 dopamine receptor binding; D5 dopamine receptor binding; protein binding; protein kinase inhibitor activity; protein phosphatase inhibitor activity; protein phosphatase type 1 regulator activity; type 1 serine/threonine specific protein phosphatase inhibitor activity Biological Process: negative regulation of catalytic activity; negative regulation of female receptivity; regulation of catalytic activity; response to amphetamine; signal transduction; transcription, DNA-dependent; visual learning |
| NCBI Summary: | This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | Q9UD71 |
| NCBI GenInfo Identifier: | 23831292 |
| NCBI Gene ID: | 84152 |
| NCBI Accession: | Q9UD71.2 |
| UniProt Secondary Accession: | Q9UD71,Q547V9, Q547W0, Q9H7G1, |
| UniProt Related Accession: | Q9UD71 |
| Molecular Weight: | 18,738 Da |
| NCBI Full Name: | Protein phosphatase 1 regulatory subunit 1B |
| NCBI Synonym Full Names: | protein phosphatase 1 regulatory inhibitor subunit 1B |
| NCBI Official Symbol: | PPP1R1B |
| NCBI Official Synonym Symbols: | DARPP32; DARPP-32 |
| NCBI Protein Information: | protein phosphatase 1 regulatory subunit 1B |
| UniProt Protein Name: | Protein phosphatase 1 regulatory subunit 1B |
| UniProt Synonym Protein Names: | DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein |
| Protein Family: | Protein phosphatase 1 regulatory |
| UniProt Gene Name: | PPP1R1B |
| UniProt Entry Name: | PPR1B_HUMAN |
 | Western blot analysis of extracts of 293T cells, using PPP1R1B antibody (CAB2580) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3s. |
 | Immunofluorescence analysis of SH-SY5Y cells using PPP1R1B Rabbit pAb (CAB2580) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |