Anti-PPM1F Antibody (CAB15363)
- SKU:
- CAB15363
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PPM1F Antibody |
| Antibody SKU: | CAB15363 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | MCF7, HeLa, HepG2, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSG APQK SSPM ASGA EETP GFLD TLLQ DFPA LLNP EDPL PWKA PGTV LSQE EVEG ELAE LAMG FLGS RKAP PPLA AALA HEAV SQLL QTDL SEFR KLPR EEEE EEED DDEE EKAP VTLL DAQS LAQS FFNR LWEV AGQW QKQV PLAA RASQ RQWL VSIH AIRN TRRK MEDR HVSL PSFN QLFG LSDP VN |
| Gene ID: | 9647 |
| Uniprot: | P49593 |
| Cellular Location: | |
| Calculated MW: | 38kDa/49kDa |
| Observed MW: | 55kDa |
| Synonyms: | PPM1F, CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2 |
| Background: | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. |
| UniProt Protein Function: | PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.16; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q11.22 Cellular Component: cytosol; protein complex Molecular Function:calmodulin-dependent protein phosphatase activity; phosphoric monoester hydrolase activity; protein binding; protein serine/threonine phosphatase activity Biological Process: negative regulation of peptidyl-serine phosphorylation; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of chemotaxis; positive regulation of focal adhesion formation; positive regulation of growth; positive regulation of stress fiber formation |
| NCBI Summary: | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49593 |
| NCBI GenInfo Identifier: | 24638458 |
| NCBI Gene ID: | 9647 |
| NCBI Accession: | P49593.3 |
| UniProt Secondary Accession: | P49593,Q96PM2, A8K6G3, B7Z2C3, |
| UniProt Related Accession: | P49593 |
| Molecular Weight: | 38,518 Da |
| NCBI Full Name: | Protein phosphatase 1F |
| NCBI Synonym Full Names: | protein phosphatase, Mg2+/Mn2+ dependent 1F |
| NCBI Official Symbol: | PPM1F |
| NCBI Official Synonym Symbols: | CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase |
| NCBI Protein Information: | protein phosphatase 1F |
| UniProt Protein Name: | Protein phosphatase 1F |
| UniProt Synonym Protein Names: | Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2 |
| Protein Family: | Protein phosphatase |
| UniProt Gene Name: | PPM1F |
| UniProt Entry Name: | PPM1F_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPM1F Rabbit pAb (CAB15363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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