Anti-PPAR gamma Antibody (CAB0270)
- SKU:
- CAB0270
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-PPAR gamma Antibody |
| Antibody SKU: | CAB0270 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPAR gamma (NP_056953.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, THP-1, SW620, Mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PPAR gamma (NP_056953.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LSVM EDHS HSFD IKPF TTVD FSSI STPH YEDI PFTR TDPV VADY KYDL KLQE YQSA IKVE PASP PYYS EKTQ LYNK PHEE PSNS LMAI ECRV CGDK ASGF H |
| Gene ID: | 5468 |
| Uniprot: | P37231 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 21kDa/54kDa/57kDa |
| Observed MW: | 57kDa |
| Synonyms: | CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, PPAR gamma, PPARG |
| Background: | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. |
| UniProt Code: | P37231 |
| NCBI GenInfo Identifier: | 545687869 |
| NCBI Gene ID: | 102120453 |
| NCBI Accession: | NP_001270028.1 |
| Molecular Weight: | 59.2kD |
| NCBI Full Name: | peroxisome proliferator-activated receptor gamma |
| NCBI Official Symbol: | PPARG |
| NCBI Official Synonym Symbols: | PPARgamma1; PPARgamma2; PPARgamma3; PPARgamma4; PPARgamma5; PPARgamma6; PPARgamma7 |
| NCBI Protein Information: | peroxisome proliferator-activated receptor gamma; hypothetical protein; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma 2; peroxisome proliferator-activated receptor gamma 3; peroxisome proliferator-activated receptor gamma 4; peroxisome proliferator-activated receptor gamma 5; peroxisome proliferator-activated receptor gamma 6; peroxisome proliferator-activated receptor gamma 7 |
| UniProt Protein Name: | |
| Protein Family: | Peroxisome proliferator-activated receptor |
| UniProt Gene Name: |
 | Western blot analysis of extracts of various cell lines, using PPAR gamma antibody (CAB0270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry of paraffin-embedded Rat heart using PPAR antibody (CAB0270) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human colon using PPAR antibody (CAB0270) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse lung using PPAR antibody (CAB0270) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using PPAR gamma antibody (CAB0270) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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